Molekularne metode u klasifikaciji bakterija

undergraduate thesis

• Title (english): Molecular metods in bacterial classification
• Author: Tajana Majić
• Mentor: Jasna Hrenović (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Molekularne metode klasifikacije bakterija su brojne. Svim metodama je zajedničko to što uključuju hibridizaciju nukleinskih kiselina soja kojeg istražujemo s drugim, dobro poznatim sojem, kako bismo odredili stupanj srodnosti. Nakon određivanja stupnja srodnosti, prema stupnju hibridizacije, bakterije možemo smjestiti u taksonomske stupnjeve. Osnovne metode klasifikacije bakterija su hibridizacija DNA-DNA, hibridizacija DNA-rRNA, hibridizacija oligonukleotidima i DNA fingerprinting. U ovom radu izložene su osnovne metode klasifikacije s naglaskom na izvođenje i razlike u metodama izvođenja eksperimenata.

Abstract (english)

Several molecular methods are applied for the classification of bacteria. These methods are based on nucleic acid hybridization of an unknown bacterial strain of interest with that of a closely related already identified strain to determine the degree of genetic complementarity. The degree of hybridization, proportional to the degree of similarity is used to establish taxonomic identification. The broadly used techniques in bacterial taxonomy are DNA-DNA hybridization, DNA-rRNA hybridization, oligonucleotide hybridization and DNA fingerprinting. Here are described some of the most fundamental techniques used in bacterial classification with an emphasis on methodological issues and differences between experimental approaches.

• Keywords: klasifikacija bakterija
• Keywords (english): bacterial classification
• Language: croatian
• URN:NBN: urn:nbn:hr:217:554692
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-19 08:26:46