Alternativno izrezivanje introna u biljaka

undergraduate thesis

• Title (english): Alternative splicing in higher plants
• Author: Vanja Tadić
• Mentor: Nataša Bauer (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Alternativno izrezivanje introna proces je kojim se iz jednog genskog transkripta dobivaju različite izoforme zrele mRNA. Prisutno je u svim višim eukariotima i ima ulogu u funkcionalnom proširenju proteoma, kao i u posttranskripcijskoj regulaciji ekspresije gena. Procesi alternativnog izrezivanja introna u biljaka nisu dovoljno istraženi i tek se očekuju brojna istraživanja na tom području sukladno s razvojem novih metoda detekcije tog procesa. Sekvencioniranje genoma A. thaliana (a i drugih biljnih vrsta u novije vrijeme) omogućilo je nova saznanja o alternativnom izrezivanju introna u biljaka, a uročnjak je biljka koja se najčešće koristi u ovim istraživanjima. U praktičnome dijelu rada navedene su neke hipotetske izoforme proteinskih produkata BPM gena uročnjaka dobivene alternativnim izrezivanjem introna zbog alternativnih donorskih i akceptorskih mjesta.

Abstract (english)

Alternative splicing is a process of producing different isoforms of mature mRNA which originate from a common locus. Alternative splicing is present in all higher eukaryotes and has roles in expanding proteome diversity and post-transcriptional regulation. Alternative splicing in higher plants has not been studied extensively and new studies are anticipated as the new tools to accurately predict and visualize alternative splicing are being developed. Sequencing of the A. thaliana genome (and of other plant species, recently) has provided new information about alternative splicing in higher plants, Arabidopsis being the most commonly used species in these researches. Here, in the practical part of this work, the BPM genes of Arabidopsis are studied and some of the hypothetical isoforms of protein products derived from alternative splicing using the alternative donor and acceptor splice sites are created.

• Keywords: alternativno izrezivanje introna; biljke
• Keywords (english): alternative splicing; plants
• Language: croatian
• URN:NBN: urn:nbn:hr:217:108910
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-20 11:29:41