Analiza kariotipa slatkovodnih rakova Astacus astacus, A. leptodactylus i Austropotamobius torrentium (Decapoda, Astacidae)

master's thesis

• Title (english): Karyological analisis of freshwater crayfish Astacus astacus, A. leptodactylus and Austropotamobius torrentium (Decapoda, Astacidae)
• Author: Margerita Mužić
• Mentor: Mirjana Pavlica (mentor)
• Mentor: Jelena Mlinarec Novosel (pomoć pri izradi, neposredni voditelj)
• Committee member: Dubravka Matković-Čalogović (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ovaj rad donosi nove informacije o kariološkim značajkama autohtonih europskih vrsta slatkovodnih rakova iz rodova Astacus i Austropotamobius (Decapoda, Astacidae). Vrsta Astacus astacus ima diploidan broj kromosoma 2n=176, vrsta A. leptodactylus ima 2n=180, a vrsta Austropotamobius torrentium ima 2n=164. Formula kariotipa za vrstu A. astacus je n=88, 52m+35sm+1a, a za vrstu A. torrentium formula kariotipa je n=87, 78m+8sm+1t. Fluorescencijsko pruganje bojom 4,6-diamidino-2-fenilindol (DAPI) pokazalo je da vrste roda Astacus posjeduju velike heterokromatinske blokove smještene centromerno na svim kromosomima, dok vrsta A. torrentium samo na nekim kromosomima. Interkalarni heterokromatinski blokovi nađeni su na nekim kromosomima u sve tri vrste. U vrstama A. astacus i A. leptodactylus sparivanje kromosoma u mejozi je pravilno, a većina bivalenata su kružnog i zvonolikog oblika. Fluorescencijska hibridizacija in situ (FISH) otkrila je da vrste A. astacus i A. leptodactylus imaju po dva lokusa za 45S rRNA. U vrsti A. leptodactylus uočen je dodatni 45S rDNA signal.

Abstract (english)

This study gives karyological information on native European species Astacus astacus, A. leptodactylus and Austropotamobius torrentium (Decapoda, Astacidae). Astacus astacus has a diploid chromosome number 2n=176, species A. leptodactylus has 2n=180 and species Austropotamobius torrentium has 2n=174 chromosomes. Karyotype formula of A. astacus is n=88, 52m+35sm+1a, while for A. torrentium the formula is n=87, 78m+8sm+1t. Fluorescence banding with 4,6-diamidino-2-phenylindol (DAPI) has shown long heterochromatic blocks in centromeric regions of all chromosomes in Astacus species, and on some chromosomes in species A. torrentium. Interstitial blocks are found in some chromosomes of all three species. Meiotic pairing of chromosomes is regular in A. astacus i A. leptodactylus, and most of bivalents are in ring and dumbbell form. Furthermore, fluorescence in situ hybridization (FISH) reveals two 45S rDNA loci in both A. astacus and A. leptodactylus. An additional 45S rDNA signal was found in A. leptodactylus.

• Keywords: Astacus; Austropotamobius; Decapoda; Astacidae; FISH; kariotip; DAPI
• Keywords (english): Astacus; Austropotamobius; Decapoda; Astacidae; FISH; kariotype; DAPI
• Language: croatian
• URN:NBN: urn:nbn:hr:217:607063
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-24 11:39:17