Upotreba sustava CRISPR/Cas u genomskom inženjerstvu

undergraduate thesis

• Title (english): Use of CRISPR/Cas system in genome-editing
• Author: Mario Stojanović
• Mentor: Ivana Ivančić Baće (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

CRISPR/Cas sustav u prokariota i arheja igra važnu ulogu u obrani od infektivnih čestica. Izučavanjem principa rada CRISPR/Cas sustava ustanovljeno je da se može iskoristiti u uređivanju kako prokariotskog tako i eukariotskog genoma. Budući da je još uvijek novitet u znanosti veliki broj znanstvenika pokazuje interes za izučavanje i poboljšanje ovog sustava. Svi dosadašnji rezultati upućuju na svijetlu budućnost u primjeni CRISPR/Cas sustava u svim granama znanosti U ovom radu opisano je na koji način se sustav CRISPR/Cas pokušalo prilagoditi da bude što efikasniji i precizniji te koje su prednosti divljeg tipa CRISPR/Cas sustava spram mutiranog. Nadalje je opisana na koji način se mogu iskoristiti svojstva divljeg tipa i mutiranog tipa Cas9 nukleaze te njegova praktična primjena u mijenjanju genoma.

Abstract (english)

CRISPR/Cas system plays important role in defence from infective particlesin Prokaryotes and Arhaea. The mechanism of CRISPR/Cas system can be used in genomeediting not only in Prokaryotes but also in Eukaryotes. Because its a novelty, a great number of scientists show interest in studying and possible improvement of this system. To date all results indicates show that in the near future the use of this system will be inevitable in various disciplines of science. This paper describes recent results of the use of CRISPR/Cas system. It also shows how it can be modified to improve its efficiency and precision and which are the advantages of then wilde type CRISPR/Cas in contrast to mutant form. Further more it is described the practical use of wild type and mutated type of Cas9 nuclease and how it can be used in genome editing.

• Keywords: prokarioti; CRISPR/Cas sustav; genom
• Keywords (english): prokaryote; CRISPR/Cas system; genome
• Language: croatian
• URN:NBN: urn:nbn:hr:217:210488
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-25 09:03:39