Morfološka i genska karakterizacija populacije velike pliske (Alburnus sarmaticus Freyhof & Kottelat, 2007) u Hrvatskoj

master's thesis

• Title (english): Morphological and genetic characterization of shemaya population (Alburnus sarmaticus Freyhof & Kottelat, 2007) in Croatia
• Author: Rebecca Konjuh
• Mentor: Ivana Buj (mentor)
• Committee member: Ivana Buj (član povjerenstva)
• Committee member: Ivana Šola (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Committee member: Davor Zanella (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-03-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Vrsta Alburnus sarmaticus u Europi obitava u rijekama Južni Bug i Dniepr u Ukrajini, rijeci Dunav u Rumunjskoj i najvjerojatnije u Bugarskoj. Jedna posve izolirana populacija nastanjuje rijeke Kupu, Mrežnicu i Dobru (Hrvatska i Slovenija). U ovo istraživanje uključene su jedinke s navedena tri lokaliteta u Hrvatskoj. Kako bi opisala morfološke i genske karakteristike ove populacije u Hrvatskoj te odredila razinu interpopulacijske i intrapopulacijske raznolikosti u istim značajkama, koristila sam morfometrijske i filogenetske analize. Morfometrijske analize su uključivale mjerenje pojedinih duljina na tijelu riba, njihovu statističku obradu i uspoređivanje. Najveću duljinu tijela imala je populacija iz Dobre, a najmanju populacija iz Kupe. Broj šipčica se razlikovao među populacijama. Najviše ljusaka u bočnoj pruzi imala je populacija iz Kupe. Analizom varijance utvrđena je razlika između uzoraka jedinki svih populacija u 18 omjera. Analizom glavnih komponenata uočeno je blago izdvajanje populacije iz Kupe i preklapanje populacija iz Dobre, Mrežnice i Dunava. Filogenetske analize provela sam metodom najveće parsimonije i metodom najveće vjerojatnosti pomoću programa MEGA. Dobivena filogenetska stabla rezultirala su sličnom topologijom i dobrom podržanosti vanjskih grananja. Sekvence velike pliske izdvojile su se u zasebnu, monofiletsku liniju, koja predstavlja sestrinsku liniju ostalim vrstama roda Alburnus i potvrđuje njihovu nezavisnu evoluciju.

Abstract (english)

Alburnus sarmaticus species is represented in Europe in the rivers South Bug and Dniepr in Ukraine, the river Danube in Romania and most likely in Bulgaria. One completely isolated population inhabits the rivers Kupa, Mrežnica and Dobra (Croatia and Slovenia). Included in this research are individuals from these three sites in Croatia. In order to describe the morphological and genetic characteristics of this population in Croatia and determine the level of interpopulation and intrapopulational diversity in genetic and morphological features, I used morphometric and phylogenetic analyzes. Morphometric analyzes included measuring specific individual lengths on the fish body and their statistical processing and comparison. The population from Dobra river had the largest body length and the population from Kupa river had the smallest. The number of spines differed among the populations. Population from Kupa river had the most scales in a lateral line. The variance analysis confirmed the difference between the samples of all populations in 18 proportions. Analysis of the main components showed a slight separation of populations from Kupa river and overlapping populations from Dobra, Mrežnica and Danube rivers. Phylogenetic analyzes were performed by the maximum parsimony and the maximum likelyhood methods using the MEGA program. Phylogenetic trees which were obtained showed a similar topology and a good support of the outer branches. Shemaya sequences made a separate, monophyletic lineage in phylograms, sister lineage to all the remaining Alburnus species, which corraborates its separate evolutionary history.

• Keywords: Alburnus sarmaticus; morfologija; meristika; filogenija; Hrvatska
• Keywords (english): Alburnus sarmaticus; morphology; meristic; phylogeny; Croatia
• Language: croatian
• URN:NBN: urn:nbn:hr:217:240936
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-03-14
• Created on: 2018-05-16 08:31:04