Changes of immunoglobulin G glycosylation with age

doctoral thesis

• Title (croatian): Promjena glikozilacije immunoglobulina G tijekom starenja
• Author: Jasminka Krištić
• Mentor: Gordan Lauc (mentor)
• Committee member: Olga Gornik (predsjednik povjerenstva)
• Committee member: Kristian Vlahoviček (član povjerenstva)
• Committee member: Igor Rudan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

In this thesis, glycosylation of immunoglobulin G (IgG) was examined in 5818 individuals ranging in age from 16 to 100 years from five different populations – four European populations and a Han Chinese population by using ultra performance liquid chromatography (UPLC) in order to provide a comprehensive overview of changes in IgG glycosylation through lifetime. The results demonstrated that glycan profiles change in a similar way through lifetime across all examined populations. Nearly all IgG glycans were significantly associated with age. Sex differences in age-related changes in IgG glycosylation were observed. The combination of several IgG glycans was able to explain from 30 to 58% of variance in chronological age, with the remaining variance in the glycans attributed to physiological parameters. Analysis of IgG glycosylation in nearly 600 mice from the Collaborative Cross cohort ranging in age from 20 to 80 weeks showed that, generally, only the level of IgG glycan with alpha-1,3-galactose changed with age.

Abstract (croatian)

U ovom doktorskom radu analizirana je glikozilacija imunoglobulina G (IgG) kod 5818 osoba starosti od 16 do 100 godina iz pet različitih populacija – četiri europske populacije i populacije Han Kineza, koristeći tekućinsku kromatografiju vrlo visoke djelotvornosti (UPLC), kako bi se pružio detaljan pregled promjena u glikozilaciji IgG-a tijekom starenja. Rezultati su pokazali da se glikanski profili tijekom starenja mijenjaju na sličan način u svim istraživanim populacijama. Gotovo svi IgG glikani pokazali su se povezanima s dobi. Uočene su razlike između spolova u promjenama koje se događaju s glikozilacijom IgG-a tijekom starenja. Kombinacijom nekoliko IgG glikana bilo je moguće objasniti od 30 do 58% varijacije u kronološkoj dobi, a ostatak varijacije u glikanima pripisan je fiziološkim parametrima. Analiza glikozilacije IgG-a kod gotovo 600 miševa starosti od 20 do 80 tjedana iz Collaborative Cross populacije miševa pokazala je da se, sveobuhvatno gledajući, samo razina IgG glikana koji sadrži alfa-1,3 vezanu galaktozu promijenila s dobi.

• Keywords: glycosylation; immunoglobulin G; chronological age; biological age; biomarker; humans; mice
• Keywords (english): glikozilacija; imunoglobulin G; kronološka dob; biološka dob; biomarker; ljudi; miševi
• Language: english
• URN:NBN: urn:nbn:hr:217:847581
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-09-04 09:49:58