Development of fractionation procedures for comprehensive characterization of organic contaminants in surface waters using effect-directed analyses

doctoral thesis

• Title (croatian): Razvoj frakcionacijskih postupaka za sveobuhvatnu karakterizaciju organskih zagađivala u površinskim vodama primjenom analiza usmjerenih učincima
• Author: Sanja Bačić
• Mentor: Marijan Ahel (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Senka Terzić (član povjerenstva)
• Committee member: Tvrtko Smital (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2019-01-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

In this work, a novel extraction and fractionation protocol for comprehensive characterization of organic contamination in surface waters using effect-directed analyses (EDA) was developed. A tiered EDA approach was applied, which included a simple EDA protocol, suitable for routine applications, and a higher-tier protocol for a detailed multidimensional fractionation with a final aim to identify key contaminants. A mixed-bed sorbent, consisting of three complementary phases was applied for the extraction, while three different approaches were applied for the fractionation. Sequential elution was found to be the most efficient approach, which provided good recoveries of as well as successful fractionation various organic contaminants according to charge. The developed EDA procedure was successfully applied for the assessment of organic toxicants in the Sava River. Application of a battery of bioassays showed a significant toxicity of Sava River samples, while target and non-target analyses revealed the presence of numerous contaminant classes. The combination of biological and chemical analyses is an important basis for the prioritization of organic contaminants in the Sava River basin.

Abstract (croatian)

U ovom radu razvijeni su ekstrakcijski i frakcionacijski postupci za sveobuhvatnu karakterizaciju organskog onečišćenja u površinskim vodama primjenom analiza usmjerenih učincima (engl. Effect-Directed Analysis, EDA). Prilikom razvoja EDA protokola primijenjen je stupnjeviti pristup koji je uključio razvoj jednostavnog protokola, pogodnog za rutinske analize, te protokola za detaljnu višedimenzionalnu frakcionaciju s ciljem konačne identifikacije kritičnih spojeva. Za ekstrakciju organskih zagađivala upotrijebljena je kombinacija triju sorbensa komplementarnih svojstava, a za frakcionaciju su uspoređena tri različita pristupa. Sekvencijalna eluacija zagađivala s miješanog sorbensa pokazala se najučinkovitijim pristupom jer uz dobra iskorištenja omogućuje i sustavno razdvajanje zagađivala u karakteristične frakcije prema naboju molekule. Razvijeni postupak primijenjen je za ekotoksikološku karakterizaciju zagađivala u rijeci Savi. Primjenom odabranih bioloških testova detektirani su značajni toksični učinci u rijeci Savi, a ciljane i pretražne kemijske analize ukazale prisutnost velikog broja zagađivala. Kombinacija rezultata bioloških i kemijskih analiza predstavlja važnu osnovu za prioritizaciju organskih zagađivala u bazenu rijeke Save.

• Keywords: bioassays; chemical analyses; EDA; fractionation procedures; organic contaminants; surface waters; Sava River
• Keywords (croatian): biološki testovi; EDA; fractionacijski postupci; kemijske analize; organska zagađivala; površinske vode; rijeka Sava
• Language: english
• URN:NBN: urn:nbn:hr:217:083275
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: 237 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-02-15 13:47:52