Alfa satelitska RNA i gen MSH2:potencijalni kandidati za dijagnostički/prognostički krvni biomarker raka prostate

master's thesis

• Title (english): Alpha satellite RNA and MSH2 gene: potential candidates for diagnostic/prognostic blood's biomarker of prostate cancer
• Author: Zrinka Matić
• Mentor: Đurđica Ugarković (mentor)
• Committee member: Đurđica Ugarković (predsjednik povjerenstva)
• Committee member: Sven Jelaska (član povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-02-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Rak prostate drugi je najčešći oblik raka kod muškaraca u Hrvatskoj. Klinički najprihvaćeniji biomarker za dijagnozu raka prostate je PSA (prostata-specifični antigen), ali zbog nedostatka njegove specifičnosti intezivni napori trenutno su usmjereni na otkrivanje alternativnih dijagnostičkih i prognostičkih biomarkera za rak prostate. Biomarker se definira kao biološka molekula iz krvi, drugih tjelesnih tekućina ili tkiva koja može biti objektivno izmjerena i evaluirana kao znak normalnog ili abnormalnog biološkog procesa i patoloških stanja/bolesti. Cilj istraživanja bio je provjeriti promjenu ekspresije dvaju predloženih kandidata iz krvi pacijenata s rakom prostate uspoređujući ih s zdravim kontrolama. Budući da je uočena smanjena ekspresija statistički značajna, alfa satelitska RNA i gen MSH2 predloženi su kao kandidati za neinvazivni dijagnostički/prognostički biomarker raka prostate.

Abstract (english)

Prostate cancer is the second most common cancer among men in Croatia. The clinically most accepted biomarker for prostate cancer diagnosis is PSA (prostate-specific antigen), but due to the lack of its specificity intensive efforts are currently directed towards a search for alternative diagnostic and prognostic prostate cancer biomarkers. Biomarker is defined as a biological molecule found in blood, other body fluids, or tissues that can be objectively measured and evaluated as a sign of a normal or abnormal biological process and a pathogenic condition/disease. The aim of this research was to check alteration of expression of two proposed biomarker candidates in the blood of prostate cancer samples compared with healthy controls. As observed downregulation was statistically significant, alpha satellite RNA and MSH2 gene are proposed as candidates for non-invasive diagnostic/prognostic biomarkers of prostate cancer.

• Keywords: krv; molekularni markeri; smanjenje ekspresije; neinvazivna metoda
• Language: croatian
• URN:NBN: urn:nbn:hr:217:382815
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-02-22
• Created on: 2019-03-27 13:32:25