Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade

master's thesis

• Title (english): Characterization of newly-formed tissue after nasal septum cartilage biopsy
• Author: Matea Brezak
• Mentor: Inga Marijanović (mentor)
• Committee member: Inga Marijanović (predsjednik povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Romana Gračan (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-02-12, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Danas se u liječenju oštećenja zglobne hrskavice ili hrskavice nosne pregrade sve češće koriste tkivni presatci dobiveni metodama tkivnog inženjerstva. Prilikom uzgoja tkivnih presadaka potreban je izvor stanica koje će se nasaditi na nosač, te se u tu svrhu uzima biopsija hrskavice nosne pregrade. Uslijed tog postupka, na mjestu biopsije ostaje praznina koja se tijekom vremena ispuni tkivom nepoznatog porijekla i morfologije. Do sada nisu provedena istraživanja na velikim modelnim organizmima koja bi dokazala posjeduje li novonastalo tkivo svojstva potrebna svojstva da bi se održala normalna funkcija pregrade. Ukoliko se formira novo hrskavično tkivo funkcionalnost ne bi bila ugrožena te bi se omogućilo ponovno uzimanje hondrocita s istoga mjesta. Cilj ovog istraživanja je putem histoloških i imunohistokemijskih bojanja, kao i analizom ekspresije gena metodom qPCR okarakterizirati novonastalo tkivo na mjestu biopsije. Histološka analiza je pokazalaniski regenerativni potencijal hrskavičnog tkiva kao i ulogu perihondrija u stvaranju novog tkiva. Analiza ekspresije gena novonastalog tkiva je potvrdila histološke rezultate.. Dodatno, visoka ekspresija gena COL 1, COL 4 i CRABP 1 potvrdila je značajnu ulogu vanjskog fibroznog sloja perihondrija u nastanku novog tkiva

Abstract (english)

Tissue engineering has become one of the most studied fields with clinical application. When producing cartilage tissue in vitro, nasal septum biopsy is taken in order to harvest chondrocytes that will be seeded on scaffolds. Following this procedure, the nasal septum is left with a perforation that fills in over time. The newly-formed tissue may not possess functional properties characteristic for septum cartilage, whereas if the neocartilage forms it would enable chondrocyte reharvesting and confirm normal septum function. To this time, no one has addressed the issue of tissue formation in the place of the perforation on a large animal model. Therefore, in this study, newly-formed tissue was characterized using histological and immunohistochemical stainings including analysis of cartilage and perichondrium marker gene expression. Low regenerative potential of cartilage was confirmed as no formation of similar tissue was observed over time. Perichondral origin of newly-formed tissue was confirmed both histologically and through gene expression analysis. Additionally, high expression of COL 1, COL 4 and CRABP 1 genes indicate a significant role of outer perichondrium layer in tissue genesis.

• Keywords: ekspresija gena; hrskavica nosnog septuma; imunohistokemijska analiza; perihondrij; tkivno inženjerstvo
• Keywords (english): gene expression; immunohistochemical analysis; nasal cartilage; perichondrium; tissue engineering
• Language: croatian
• URN:NBN: urn:nbn:hr:217:798467
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-02-12
• Created on: 2019-04-02 10:20:29