Evolucija ključnih peroksisomalnih proteina

master's thesis

• Title (english): Evolution of core peroxisomal proteins
• Author: Hrvoje Mišetić
• Mentor: Toni Gabaldon (mentor)
• Mentor: Damjan Franjević (mentor)
• Committee member: Damjan Franjević (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Silvija Černi (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-02-08, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Postoji niz neodgovorenih pitanja o evolucijskom podrijetlu peroksisoma. Prije desetak godina provedena je filogenetska analiza peroksisomalnog proteoma u kojoj je otkriven minimalni ancestralni peroksisomalni proteom. Današnja velika količina genomskih podataka pruža idealne uvjete za analizu podrijetla ancestralnog peroksisomalnog proteoma. Kako bi se riješilo to pitanje, filogenetska stabla su napravljena za svaki protein u proteomu i nadopunjena su predikcijama sub-stanične lokalizacije i informacijama o proteinskim domenama s namjerom da se provede detaljna filogenetska analiza. Peroksini Pex1, Pex2, Pex4 i Pex10 imaju zajedničko podrijetlo s komponentama sustava ERAD, Pex5 s komponentama kompleksa koji promovira anafazu (engl. anaphase promoting complex) dok Pex14 nema evolucijsku povezanost s nijednim molekularnim sustavom. Pxa1 i Pxa2 koji imaju ulogu u transportu masnih kiselina imaju neovisno podrijetlo od odgovarajućih proteina u mitohondriju i staničnoj membrani. Dodavanje predstavnika nedavno sekvenciranih eukariotskih koljena i članova Asgard grupe ne mijenja postojeću filogeniju katalaze. Fox1 i Faa2 koji imaju ulogu u peroksisomalnoj β-oksidaciji masnih kiselina imaju neovisno podrijetlo od proteina koji sudjeluju u istom procesu smještenom u mitohondriju-dok Fox2 koji katalizira drugi korak oksidacije masnih kiselina ima podrijetlo iz mitohondrija.

Abstract (english)

There are a lot of unanswered questions about the evolutionary origin of the peroxisome. About ten years ago a phylogenetic analysis of peroxisomal proteome was conducted during which the minimal ancestral eukaryotic peroxisomal proteome was discovered. These days the larger amount of genomic data provides an ideal framework to analyze the origin of the ancestral peroxisome. To address this question, phylogenetic trees for each protein from the proteome were reconstructed and complemented with predictions of subcellular localization and protein domain information to perform a detailed phylogenetic analysis. Peroxins Pex1, Pex2, Pex4 and Pex10 show common origin with the components of ERAD, Pex5 with components of anaphase promoting complex/cyclosome (APC/C) while Pex14 doesn’t have evolutionary relationship with any molecular system. Pxa1 and Pxa2 that are involved in lipid transport show independent origin from its mitochondrial and cell membrane counterparts. Adding members from newly sequenced eukaryotic phyla and Asgard group didn’t change previously known phylogeny of catalase. Fox1 and Faa2 that are involved with peroxisomal β-oxidation show independent origin from the same process in mitochondria while Fox2 that catalyzes second step of β-oxidation is probably derived from mitochondria.

• Keywords: peroksisom; evolucija; filogenomika
• Language: croatian
• URN:NBN: urn:nbn:hr:217:704925
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-08
• Created on: 2019-04-02 10:45:35