Uloga peptida BPC 157 u cijeljenju mišićno-tetivne sveze mišića kvadricepsa u štakora

doctoral thesis

• Title (english): The role of the peptide BPC 157 in healing muscle-tendon junction in the quadriceps muscle in rat
• Author: Mladen Japjec
• Mentor: Predrag Sikirić (mentor)
• Mentor: Božidar Šebečić (komentor)
• Committee member: Aleksandar Vojta (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-03-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Svakodnevno, a posebno tijekom sportskih aktivnosti česta su oštećenja mišića i tetiva. Mišićno-tetivni spoj je najslabija komponenta u cijeloj jedinici i veliki broj oštećenja dogaĎa se na njemu. Na temelju rezultata ranijih istraţivanja u kojima terapija BPC 157 dovodi do cijeljenja oštećene tetive ili mišića, postavili smo hipotezu da BPC 157 utječe pozitivno na cijeljenje i ponovnu uspostavu čvrste i kvalitetetne mišićno-tetivno sveze mišića kvadricepsa u štakora. Eksperimentalnim ţivotinjama, s kirurški razdvojenim mišićnim od tetivnih niti u području četveroglavog mišića desne natkoljenice raspodjeljenim u 3 skupine davali smo: BPC 157 u dozi od 10 ng/kg, BPC 157 u dozi od 10 μg/kg ili fiziološku otopinu. Pratili smo kliničko-fiziološke promjene, a po ţrtvovanju napravili makroskopske i histološke analize te biomehanička i laboratorijska testiranja tkiva. U patohistološkim preparatima obje skupine BPC tretiranih ţivotinja u odnosu na kontrolnu, bila je značajno manja upala te puno brţa i kvalitetnija reparacija mišićno-tetivog spoja. Cijeljenje je bilo popraćeno većom ekspresijom eNOS mRNA i smanjenom razinom NO i slobodnih radikala kod jedinki koje su tretirane BPC 157 u odnosu na kontrolnu skupinu. Isto je rezultiralo manjim razvojem atrofije mišića i nastajanja defekta na mjestu spoja. Tretirane ţivotinje bolje su hodale, imale jaču snagu pritiska i nisu razvijale kontrakturu noge. Naši rezultati pokazuju da bi se BPC 157 mogao koristiti kod ozljeda mišićno-tetivne sveze obzirom da poboljšava cijeljenje, a za kliničku praksu neophodna su još dodatna istrazivanja.

Abstract (english)

Daily and especially during sports activities muscle and tendon are often damaged. How is the muscular tendon junction weakest component in the entire unit so that a large number of defects occurring right on that part. According to the results of previous studies in which therapy BPC 157 leads to successful healing of injuries isolated tendon or muscle tissue, we hypothesized that BCP157 positively affects on the healing and restoration of solid and quality muscular-tendon connection. Experimental animals with surgical separate muscle from tendon in the right muscle quadriceps and randomly devided into the 3 groups, will be treated parenterally with: BPC 157 at a dose of 10 ng/kg, BPC 157 at a dose of 10 μg/kg or saline. During research, we followed clinical-physiological changes and after sacrificing macroscopic, histological and biomechanical tissue testing were done. In the pathohistological preparations of both groups of BPC treated animals in comparison to the control, there was noticeably less inflammation and a much faster and better repair of the muscle-tendon join. Healing was followed with greater expression of the eNOS mRNA and reduced levels of NO and free radicals. This has resulted, macroscopically, with the lower development of muscle atrophy and the formation of a defect in the muscle-tendon junction. Treated animals walked better, they had stronger pressure with leg and the contractures did not developed. The contribution of labor would represent unquestionable novelty in healing muscle-tendon junction.

• Keywords: mišićno-tetivna sveza; BPC 157; NO sustav; cijeljenje; štakori
• Keywords (english): muscle-tendon junction; BPC 157; NO system; healing; rats
• Language: croatian
• URN:NBN: urn:nbn:hr:217:693935
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-03-29
• Created on: 2019-04-10 08:52:52