The role of bridging fibers in the architecture of the metaphase mitotic spindle

doctoral thesis

• Title (croatian): Uloga premošćujućih vlakana u arhitekturi metafaznoga diobenoga vretena
• Author: Bruno Polak
• Mentor: Inga Marijanović (mentor)
• Committee member: Juraj Simunić (predsjednik povjerenstva)
• Committee member: Marin Barišić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Mitosis is a fundamental process during which the genetic material is transferred to
daughter cells and is orchestrated by the mitotic spindle. Between two opposite spindle poles
microtubules form bundles that interact with chromosomes. Bundles that bind to kinetochores on
the chromosome are called kinetochore fibers, whilst certain microtubules form antiparallel
overlap bundles in the central part of the spindle. With this research localization and distribution
of antiparallel overlap bundles in metaphase and the spatial organization of these bundles and
kinetochore fibers possess will be investigated. By using confocal microscope whole metaphase
spindles will be imaged which will enable to determine the number of overlap regions and their
association with kinetochore fibers. Further work will be focused on understanding the spatial
organization of the whole mitotic spindle. Used approach revealed that nearly all antiparallel fibers
are linked with kinetochore fibers and that human mitotic spindle is a chiral structure.

Abstract (croatian)

Mitoza je fundamentalan process kojim se genetička informacija prenosi na stanice kćeri,
a struktura koja upravlja ovim procesom je diobeno vreteno, Između dva nasuprotna pola vretena
pružaju se vlakna mikrotubula koja stvaraju interakcije sa kromosomima. Vlakna koja se vežu za
kinetohore na kromosomima nazivaju se kinetohorna vlakna. Neki od ostalih svežnjeva stvaraju
antiparalelne preklapajuće regije u centralnom djelu diobenog vretena. U ovom radu ispitati će se
lokalizacija i zastupljenost antiparalelnih preklapajućih regija u metafazi te će se istražiti prostorna
organizacija navedenih i kinethornih vlakana. Uz pomoć metode konfokalne mikroskopije snimati
će se cijela vretena što će omogućiti utvrđivanje broja antiparalelnih svežnjeva, a odgovoriti će na
pitanje njihove povezanosti sa kinetohornim vlaknima. Daljnji rad biti će usmjeren na
razumijevanje prostorne organizacije metafaznog diobenog vretena. Uz primjenu navedenog
pristupa, otkriveno je da su antiparalelna vlakna povezana sa kinetohornim vlaknima te da je
diobeno vreteno u ljudskim stanicama kiralna struktura.

• Keywords: mitosis; mitotic spindle; metaphase; microtubule; bridging fiber
• Keywords (croatian): mitoza; diobeno vreteno; metafaza; mikrotubuli; premošćujuća vlakna
• Language: english
• URN:NBN: urn:nbn:hr:217:642253
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-07-16 09:40:27