Procjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma

master's thesis

• Title (english): Toxicity assessment of Sava river using onion (Allium cepa L.) as a model organism
• Author: Iva Kokotović
• Mentor: Sandra Radić Brkanac (mentor)
• Committee member: Sandra Radić Brkanac (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Povećana urbanizacija i otpuštanje otpadnih voda u rijeke dovodi do sve većeg broja organskih zagađivača u vodenim ekosustavima i potrebe za pročišćavanjem otpadnih voda. Kvaliteta vode rijeke Save, uzorkovane na četiri lokacije (Lukavec, Rugvica, Jesenice i Hrušćica), u ovom je radu procijenjena pomoću Allium-testa i biokemijskih parametara - sadržaja proteina, karbonila i malondialdehida te aktivnosti antioksidacijskih enzima (superoksid dismutaze, askorbat peroksidaze, katalaze i glutation-S-transferaze). Kao modelni organizam korišten je obični luk (Allium cepa L.), koji je pogodan za istraživanje cito- i genotoksičnosti zbog malog broja dugačkih kromosoma (2n = 16). Uzorci vode rijeke Save nisu inhibirali rast korjenčića luka, no pokazali su se genotoksičnim, a uzorak s lokacije Jesenice i citotoksičnim. Rezultati aktivnosti antioksidacijskih enzima i karbonilacije proteina pokazuju da su svi uzorci vode rijeke Save izazvali oksidacijski stres u korjenčićima luka i posljedično uzrokovali oštećenja na proteinima. Zbog jednostavnog načina čuvanja i rukovanja te osjetljivosti, obični luk je pogodan modelni organizam za biomonitoring površinskih voda.

Abstract (english)

Increased urbanization and disposal of wastewater into rivers leads to ever-growing number of organic contaminants in aquatic ecosystems and the need for wastewater treatment. In the present study, the quality of Sava River collected at four locations (Lukavec, Rugvica, Jesenice i Hrušćica) has been evaluated using Allium-test and certain biochemical parameters – contents of proteins, malondialdehide and carbonyls as well as activity of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, catalase and glutathione-S-transferase). Onion (Allium cepa L.) is often used as a model organism in cyto- and genotoxic studies because it has a small number of relatively large chromosomes (2n = 16). Samples of Sava River did not cause growth inhibition of onion roots, but they were genotoxic and the sample Jesenice was cytotoxic as well. The results of antioxidative enzyme activities and protein carbonilation showed that all samples of Sava River cause oxidative stress in onion roots and consequently damage to proteins. Due to the simplicity and low cost of the procedure as well as its sensitivity, onion is a suitable model organism for biomonitoring of surface waters.

• Keywords: Allium cepa L.; Allium-test; oksidativni stres; antioksidacijski enzimi; organski zagađivači
• Keywords (english): Allium cepa L.; Allium-test; oxidative stress; antioxidant enzymes; organic pollutants
• Language: croatian
• URN:NBN: urn:nbn:hr:217:439350
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-09-19
• Created on: 2019-10-09 11:23:18