Uloga polimorfizama gena BIRC5 (survivin) u različitim tipovima tumora jajnika

master's thesis

• Title (english): The role of BIRC5 (survivin) polymorphisms in different types of ovarian tumors
• Author: Tajana Majić
• Mentor: Vesna Musani (mentor)
• Mentor: Inga Marijanović (mentor)
• Committee member: Inga Marijanović (predsjednik povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Committee member: Damjan Franjević (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Gen BIRC5 i njegov proteinski produkt survivin imaju važnu ulogu u procesu apoptoze. U zdravim tkivima survivin je eksprimiran samo tijekom embrionalnog razvoja dok u diferenciranim tkivima odraslih organizama nije eksprimiran. Eksprimiran je u mnogim tumorima i njegova ekspresija je povezana s nastankom tumora, njihovim povratkom i lošijim preživljenjem. Istraživala sam polimorfizme u promotorskoj regiji, eksonu 4 te 3' UTR regiji gena BIRC5 kod različitih tipova tumora jajnika metodom analize heterodupleksa i sekvenciranjem DNA. Pronašla sam 15 polimorfizama od kojih c.9809T>C ima statističku značajnost u karcinomima u usporedbi s kontrolama, a c.-683C>T je novootkriven. Učestalosti ostalih polimorfizama odgovaraju referentnim vrijednostima. Čini se da polimorfizmi BIRC5 nemaju značajnu ulogu u nastanku tumora jajnika osim možda u slučaju c.9809T>C, no moguće je da bi veći uzorak pokazao drugačije rezultate.

Abstract (english)

BIRC5 gene and its protein product survivin has an important role in the process of apoptosis. In healthy tissues survivin is expressed only during embryonal development but it is not expressed in differentiated tissues of adult organisms. It is expressed in many tumors and its expression is linked to development of tumors, their recurrence and lower survival. I studied polymorphisms in promoter region, exon 4 and 3'UTR region of BIRC5 gene in different types of ovarian tumors using heteroduplex analysis method and DNA sequencing. I found 15 polymorphisms out of which c.9809T>C has statistical significance in cancer samples compared to controls, and c.-683C>T is newly discovered. Frequency of other polymorphisms match reference values. It appears that BIRC5 polyphormisms do not have a significant role in developing ovarian tumors apart from c.9809T>C, but it is possible that a larger sample would show different results.

• Keywords: polimorfizmi; promotor; tumori; survivin
• Keywords (english): polymorphisms; promoter; tumors; survivin
• Language: croatian
• URN:NBN: urn:nbn:hr:217:223654
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 06:56:51