Učinak primjene pentadekapeptida BPC 157 u atrofiji mišića nakon resekcije patelarnoga ligamenta na modelu štakora

doctoral thesis

• Title (english): The effect of applying pentadecapeptide BPC 157 in muscle atrophy after resection of patellar ligament in a rat model
• Author: Nikica Knez
• Mentor: Predrag Sikirić (mentor)
• Mentor: Aljoša Matejčić (komentor)
• Committee member: Ivan Bojanić (predsjednik povjerenstva)
• Committee member: Zoran Tadić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Atrofija mišića je neminovna pri imobilizaciji ekstremiteta. Definitivno smanjuje izglede za dobrim ishodom liječenja. Radi se uglavnom o reverzibilnom oštećenju mišića koje u većini slučajeva rezultira samo produženom rehabilitacijom, međutim, nije rijedak slučaj da dovodi do trajne smanjene funkcije ekstremiteta.
S obzirom da ne postoji za sada farmakoterapija koja bi spriječila ili umanjila razvoj atrofije mišića mi smo postavili hipotezu da pentadekapeptid BCP157 preko NO sustava sprečava razvoj atrofije te kasnije pospješuje regeneraciju mišića, imajući u vidu ranija istraživanja o resekciji i natučenju mišića, resekciji tetive i ligamenta, cijeljenju rana te protuupalnom djelavnju pentadekapeptida BCP 157.
Štakore sa reseciranim patelarnim ligamentom na stražnjoj lijevoj nozi smo tretirali peroralno i parenteralno s BPC157 (10ug/kg), L-NAME (5 mg/kg) i L-argininom (100 mg/kg) pojedinačno i/ili u kombinaciji.
Imajući u vidu da kod reseciranog patelarnog ligamenta dolazi do atrofije mišića, a shodno prethodnim našim radovima, mjerili smo učinak pentadekapeptida BPC 157 tako što smo činili kliničko-funkcionalne testove i histološki analizirali promjene u mišiću.
Pomoću RT-PCR-a su istraživane razine eNOS, iNOS, COX-2 mRNA iz tkiva mišića. Doprinos rada (definiranje opsežnosti razvoja atrofije te sprečavanje iste, te benefitni učinak BPC 157) predstavljat će značajnu znastvenu novost.

Abstract (english)

Muscle atrophy is inevitable in limb immobilization. Definitely reduces the chances of a good outcome. It is usually about reversible muscle damage, which in most cases results in only prolonged rehabilitation, however, is not a rare case that leads to a permanently reduced extremity function.
Given that there is no pharmacotherapy that would prevent or reduce the development of muscle atrophy, we hypothesized that pentadecapeptide BCP157 in relation to NO system reduces the development of atrophy and subsequently promotes muscle regeneration, taking into account previous studies of muscle resection, tendon and ligament resection, wound healing and favorable antiinflammatory effects of pentadecapeptide BCP 157.
Rats with a resected patellar ligament on the back left leg will be treated with oral and parenteral BPC157 (10ug/kg, 10ng/kg), L-NAME (5 mg / kg) and L-arginine (100 mg / kg) alone and / or in combination.
Having in mind that the resection of patellar ligament causes muscle atrophy, according to our previous work, we will measure the effects of pentadecapeptide BPC 157, we will do clinical functional tests and histological analysis of the lesions in the muscle.
RT-PCR will investigated levels of eNOS, iNOS, COX-2 mRNA from muscle tissue. The contribution of study will be in defining comprehensive development of atrophy and prevention of the same, and benefit effects of BPC 157 which will provide a significant scientific novelty.

• Keywords: atrofija mišića; BPC 157; NO sustav; cijeljenje; štakori
• Keywords (english): muscle atrophy; BPC 157; NO system; healing; rats
• Language: croatian
• URN:NBN: urn:nbn:hr:217:718110
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-09-24
• Created on: 2019-10-22 08:29:22