Populacijska analiza atlantske plavoperajne tune, Thunnus thynnus (Linnaeus, 1758) u kaveznom uzgoju primjenom mikrosatelitnih lokusa

doctoral thesis

• Title (english): A population analysis of cage-reared atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758) by application of microsatellite loci
• Author: Radonić Ivana
• Mentor: Ivona Mladineo (mentor)
• Mentor: Ana Galov (komentor)
• Committee member: Helena Ćetković (predsjednik povjerenstva)
• Committee member: Perica Mustafić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Atlantska tuna Thunnus thynnus je pelagična riba s visokim stupnjem eksploatacije i gospodarskog značaja. Kavezni uzgoj ove vrste koji obuhvaća ulov juvenilnih divljih jedinki, njihov transport do uzgajališta i prilagodbu na kavezne uvjete života, može biti stresan za juvenilne ribe i rezultirati neočekivanim mortalitetima.
U ovom istraživanju razvijen je novi set od 13 EST-SSR za T. thynnus kako bi se utvrdilo može li se preživljavanje jedinki kroz uzgojni ciklus pratiti na genetičkoj razini i povezati s genima povezanima s imunitetom i odgovorom na uvjete stresa.
Zabilježena je niska FST vrijednost (0,005), slična frekvencija alela i niska stopa gubitka alela između uzorkovanih populacija. Dva različita pristupa za identifikaciju lokusa pod selekcijom nisu ukazala na odstupanje novorazvijenih lokusa od neutralnosti, te rezultati istraživanja upućuju na to da se oni ne mogu smatrati informativnim u istraživanom kontekstu.

Abstract (english)

Atlantic bluefin tuna Thunnus thynnus is pelagic fish with a high level of exploitation and economic importance. The capture-based aquaculture that involves the catch of wild individuals, a slow transport to the rearing facilities and adaptation to the captive environment, could be stressful for juvenile fish, consequently resulting in unexpected mortalities.
In this study, a new set of 13 EST-SSRs for T. thynnus was developed to explore whether the survival success through the farming cycle might be monitored at the genetic level and linked to several immunity and stress response-associated genes.
The observed FST value was low (0.005), showing similar allele frequencies and no major allele loss between tested populations. Two different approaches for the identification of loci under selection did not indicate departure from neutrality for any of the 13 EST-SSRs, suggesting that they may not be considered informative in the studied context.

• Keywords: Atlantska plavoperajna tuna; mikrosateliti; EST-SSR; akvakultura; Jadransko more
• Keywords (english): Atlantic bluefin tuna; microsatellites EST-SSRs; aquaculture; Adriatic Sea
• Language: croatian
• URN:NBN: urn:nbn:hr:217:231345
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-10-22 08:36:30