Filogenetski odnosi unutar roda Aurinia Desv. (Brassicaceae) utvrđeni analizom regije ndhF kloroplastne DNA

master's thesis

• Title (english): Phylogenetic relationships within genus Aurinia Desv. (Brassicaceae) inferred from chloroplast ndhF sequence data
• Author: Paolo Bartolić
• Mentor: Zlatko Liber (mentor)
• Committee member: Zlatko Liber (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Goran Kovačević (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Aurinia Desv. je rod unutar tribusa Alysseae (Brassicaceae) rasprostranjen poglavito na Balkanskom i Apeninskom poluotoku. Vrste roda Aurinia su višegodišnje biljke s pokrovom od zvjezdasto razgranjenih dlaka, listovima rozete izveruganog ili zupčastog ruba, uspravnih stabljika te grozdastim cvatovima sastavljenim od cvjetova isključivo žute boje. Cilj ovog diplomskog rada je bio odrediti srodstvene odnose unutar roda Aurinia usporedbom sekvenci kodirajuće regije ndhF kloroplastne DNA. Iz sakupljenih uzoraka lisnog tkiva, a koji su još na terenu osušeni u vrećicama sa silika-gelom, izolirana je ukupna stanična DNA. Regija ndhF kloroplastne DNA je umnožena lančanom reakcijom polimerazom (PCR), pročišćena i sekvencirana. Filogenetske analize (Bayesovska i metoda maksimalne štedljivosti) i analiza haplotipova potvrdile su monofiliju roda pri čemu su se svi analizirani uzorci grupirali u tri skupine u skladu s geografskim položajem, a ne s dosadašnjom taksonomijom. Vrsta A. saxatilis je bila genetički najraznolikija dok su amfi-jadranske vrste A. leucadea i A. sinuata bile genetički najsrodnije. Grčki endemi, A. gionae i A. moreana, su toliko srodni s vrstom A. saxatilis da ne zaslužuju taksonomski rang zasebnih vrsta. Genetska raznolikost vrsta A. corymbosa i A. petraea ukazuje na to da su one preživjele pleistocenske klimatske oscilacije u više neovisnih mikrorefugija.

Abstract (english)

Aurinia Desv. is a genus within the tribus Alysseae (Brassicaceae), distributed mainly in the Balkan and Apennine Peninsula. Species of this genus are perennial plants with an indumentum of stellate hairs, sinuate or dentate rosette leaves, grooved stems with thickening bases, and raceme inflorescences composed of exclusively yellow flowers. The aim of this thesis was to determine the relationships within the genus Aurinia by comparing the sequences of the ndhF region of chloroplast DNA. Total cellular DNA was isolated from the collected tissue samples, which were dried immediately in the field using plastic bags filled with silica-gel. The ndhF region of chloroplast DNA was amplified by polymerase chain reaction, purified and sequenced. Phylogenetic analyses (Bayesian and Maximum Parsimony methods) and haplotype network confirmed the monophyly of the genus, while all the analysed samples were grouped into three groups according to geographical location, not the current taxonomy. The species A. saxatilis was genetically most diverse, while the amphi-Adriatic species A. leucadea and A. sinuata were genetically the most related. The Greek endemic species, A. moreana and A. gionae, have proven to be so closely related to A. saxatilis that they do not deserve the taxonomic rank of separate species. The genetic diversity of the species A. corymbosa and A. petraea indicated that they survived Pleistocene climate oscillations in multiple independent microrefugia.

• Keywords: Aurinia; Brassicaceae; phylogeny; ndhF.
• Keywords (english): Aurinia; Brassicaceae; phylogeny; ndhF
• Language: croatian
• URN:NBN: urn:nbn:hr:217:360444
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-10-23 07:10:57