Povezanost polimorfizma 13. introna gena za monoamin-oksidazu tipa B i njezine aktivnosti u trombocitima sa simptomima shizofrenije

master's thesis

• Title (english): Association of monoamine oxidase type B intron 13 polymorphism and its activity in platelets with symptoms of schizophrenia
• Author: Lea Pašalić
• Mentor: Gordana Nedić Erjavec (mentor)
• Committee member: Aleksandra Maršavelski (predsjednik povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Committee member: Morana Dulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Neurobiološka podloga shizofrenije uključuje promjene dopaminergičkog sustava za čiju normalnu funkciju je odgovoran i enzim monoamin-oksidaza B (MAO-B). Cilj rada bio je istražiti postojanje povezanosti jačine simptoma shizofrenije s polimorfizmom 13. introna gena za MAO-B te s njezinom aktivnošću u trombocitima. Studija je uključila 399 ispitanika oba spola oboljelih od shizofrenije čiji simptomi su procijenjeni ljestvicom pozitivnih i negativnih simptoma (PANSS). Genotipizacija je provedena lančanom reakcijom polimeraze u stvarnom vremenu, a aktivnost trombocitne MAO-B mjerena je spektroflourimetrijski. Pronađena je povišena aktivnost trombocitne MAO-B u muškaraca s izraženijim ukupnim i negativnim te simptomima koji se odnose na kogniciju. Jačina pozitivnih, općih i psihotičkih simptoma je povezana s povišenom aktivnošću trombocitne MAO-B samo u muškaraca pušača. Nema povezanosti intenziteta simptoma s aktivnošću trombocitne MAO-B kod žena, kao ni s polimorfizmom 13. introna gena MAO-B kod muškaraca i žena. Aktivnost trombocitne MAO-B odražava intenzitet pojedinih PANSS simptoma kod muškaraca oboljelih od shizofrenije.

Abstract (english)

The neurobiological background of schizophrenia involves changes in the dopaminergic system whose normal function also depends on the enzyme monoamine oxidase B (MAO-B). The aim of this study was to investigate the relationship between the severity of schizophrenia symptoms with the MAO-B intron 13 polymorphism and platelet MAO-B activity. The study included 399 schizophrenia subjects evaluated by the Positive and Negative Syndrom Scale (PANSS). Genotyping was performed by real-time polymerase chain reaction, while platelet MAO-B activity was measured spectrofluorimetrically. Increased platelet MAO-B activity was found in men with more pronounced total, negative and cognitive symptoms. The severity of positive, general and psychotic symptoms is associated with increased platelet MAO-B activity only in male smokers. There is no association of symptoms' intensities with platelet MAO-B activity in women, nor with the MAO-B intron 13 polymorphism in men and women. Platelet MAO-B activity reflects the intensity of specific PANSS symptoms in men with schizophrenia.

• Keywords: monoamin-oksidaza tipa B; ljestvica pozitivnih i negativnih simptoma; polimorfizam 13. introna gena MAO-B; shizofrenija
• Keywords (english): monoamine oxidase B; Positive and Negative Syndrome Scale; MAO-B intron 13 polymorphism; schizophrenia
• Language: croatian
• URN:NBN: urn:nbn:hr:217:050364
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-10-23 12:26:17