Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff.

master's thesis

• Title (english): Influence of salinity and osmotic stress on protein glycosylation in Mammillaria gracilis Pffeif.
• Author: Ines Lambert
• Mentor: Biljana Balen (mentor)
• Mentor: Petra Cvjetko (mentor)
• Committee member: Sandra Radić Brkanac (predsjednik povjerenstva)
• Committee member: Damir Sirovina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ovom su radu uspoređivani utjecaji solnog i osmotskog stresa na proteine izolirane iz kalusnog i tumorskog tkiva kaktusa vrste Mamillaria gracilis Pfeiff. uzgajanih u uvjetima in vitro. Budući da se radi o biljci sa CAM metabolizmom, koja je prilagođena na sušne uvjete staništa i osjetljiva na promjene u okolišnim čimbenicima, ona je prikladan modelni organizam za proučavanje mehanizama u biljnim stanicama koji se aktiviraju tijekom izlaganja biljaka solnom i osmotskom stresu. U ovom je istraživanju pokazano kako izlaganje tkiva vrste M. gracilis soli i manitolu mijenja ekspresiju ukupnih topivih staničnih proteina i izaziva promjene u njihovoj N-glikozilaciji, važnoj posttranslacijskoj modifikaciji proteina. Osmotski stres izazvan manitolom imao je jači utjecaj na N-glikozilaciju proteina kalusa, dok je na tumorsko tkivo veći utjecaj imao solni stres (NaCl). S obzirom da je uočena razlika u ekspresiji između glikoproteina kalusnog i tumorskog tkiva nakon izloženosti istom tipu abiotičkog stresa, može se zaključiti da je N-glikozilacija proteina specifična za određeni tip tkiva. S druge strane, razlika u ekspresiji glikoproteina unutar istog tipa tkiva, nakon izloženosti različitim vrstama stresora u podlozi (NaCl ili manitol), navodi na zaključak da stupanj glikozilacije ovisi o vrsti stresa kojoj je tkivo izloženo.

Abstract (english)

In this paper, the effects of salt and osmotic stress on proteins isolated from callus and tumour tissue lines of Mamillaria gracilis Pfeiff. cacti, grown in vitro, were compared. Because it is a plant with CAM metabolism adapted to arid habitats and sensitive to changes in environmental factors, it is a suitable model organism to study the mechanisms in plant cells that are activated during plant exposure to salt and osmotic stress. In this study, it was shown that the exposure of M. gracilis tissues to salt and mannitol alters the expression of total soluble cellular proteins and induces changes in protein N-glycosylation, a very important posttranslational modification. Osmotic stress induced by mannitol had stronger impact on callus protein N-glycosylation, while tumour tissue was found to be more sensitive to salt stress (NaCl). Difference in glycoprotein expression, which was found between callus and tumour tissue after their exposure to the same type of abiotic stress, brings to conclusion that N-glycosylation is specific to certain tissues type. On the other hand, difference in glycoprotein expression, detected within the same tissue type after exposure to different stressors (NaCl or mannitol), implies that level of protein N-glycosylation is dependent on the type of the stressor.

• Keywords: Mammillaria gracilis Pfeiff.; solni stres; osmotski stres; N-glikozilacija
• Keywords (english): Mammillaria gracilis Pfeiff.; salt stress; osmotic stress; N-glycosylation
• Language: croatian
• URN:NBN: urn:nbn:hr:217:679665
• Study programme: Title: Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra edukacije biologije (magistar/magistra edukacije biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-20 08:01:37