Morphology and molecular phylogeny of the genus Branchiobdella Odier, 1823 (Annelida: Clitellata) in Croatia

master's thesis

• Title: Morfološke i molekularno-filogenetske značajke roda Branchiobdella Odier, 1823 (Annelida: Clitellata) u Hrvatskoj
• Author: Ivana Šarić
• Mentor: Ivana Maguire (mentor)
• Mentor: Goran Klobučar (mentor)
• Committee member: Ivana Maguire (predsjednik povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Goran Klobučar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014-06-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Branhiobdele su skupina holarktički rasprostranjenih malih kolutićavaca koji žive kao epibionti na slatkovodnim rakovima iz porodica Astacidae i Cambaridae. U Hrvatskoj nalazimo šest vrsta branhiobdela, koje sve pripadaju rodu Branchiobdella, te žive kao epibionti na vrstama rakova Austropotamobius pallipes, Austropotamobius torrentium i Astacus astacus. Cilj ovog istraživanja bio je morfološka determinacija vrsta branhiobdela s rakova iz roda Austropotamobius te utvrđivanje molekularnofilogenetskih odnosa između vrsta branhiobdela. Na rakovima roda Austropotamobius sa 74 lokaliteta utvrđene su sljedeće vrste branhobdela: Branchiobdella astaci, Branchiobdella hexodonta, Branchiobdella parasita, Branchiobdella pentodonta i Branchiobdella italica. Utvrđeno je 50 novih lokaliteta na kojima su pronađene branhiobdele na rakovima roda Austropotamobius u Hrvatskoj. Molekularno–filogenetski odnosi između vrsta roda Branchiobdella u Hrvatskoj utvrđeni su genskim markerima za podjedinicu 1 citokrom C oksidaze (COI) i 16S rRNA. Analizom odnosa između i unutar pojedinih taksona na filogenetskom stablu i pomoću izračuna p-distanci, zaključeno je kako je moguće da su procesi koji su doveli do specijacije unutar roda Austropotamobius također utjecali i na specijaciju samih branhiobdela.

Abstract (english)

Branchiobdellidans are ectosymbiotic annelids that are primarily associated with freshwater crayfish from the Astacidae and Cambaridae families. They have a Holartic distribution. Six species of branchiobdellidans have been reported in Croatia, all of them belong to the Branchiobdella genus. Three native Croatian species of crayfish have been reported as hosts: Austropotamobius pallipes, Austropotamobius torrentium and Astacus astacus. The objectives of this research were to morphologically identify the branchiobdellidan species living on crayfish from the Austropotamobius genus and to infer a molecular phylogeny between them. The examination of crayfish from 74 water body sites revealed the existence of five branchiobdellidan species: Branchiobdella astaci, Branchiobdella hexodonta, Branchiobdella parasita, Branchiobdella pentodonta and Branchiobdella italica. Branchiobdellidan presence has been identified in fifty new locations in Croatia. Nucleotide sequences of two mitochondrial genes (COI and 16S rRNA) have been used to estimate phylogenetic relationships between the species from the genus Branchiobdella. The results of the phylogenetic analyses, based on the examination of the constructed phylogenetic trees and values of p-distances, indicate that the same process that led to the speciation of the genus Austropotamobius in Croatia, also caused the speciation of studied branchiobdellidans.

• Keywords: Austropotamobius; branhiobdele; epibionti; p-distance; rasprostranjenost; COI; 16S rRNA
• Keywords (english): Austropotamobius; branchiobdellidans; distribution; epibionts; p-distance; COI; 16S rRNA
• Language: croatian
• URN:NBN: urn:nbn:hr:217:491288
• Study programme: Title: Eksperimentalna biologija; Study programme type: sveučilišni; Study level: diplomski; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 12:51:22