Učinci vazodilatacijskih i antikoagulacijskih lijekova na oksidativni stres u mozgu štakora

master's thesis

• Title (english): The effects of vasodilators and anticoagulants on the oxidative stress levels of the rat brain
• Author: Jure Krasić
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Marko Miliša (član povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Prethodna toksikološka istraživanja ukazuju da su krvno moždana barijera i cirkulacija središnjeg živčanog sustava vrlo osjetljivi na učinke lijekova s vazodilatacijskim ili antikoagulativnim svojstvima. Na staničnoj razini, dobro su opisani štetni učinci oksidativnog stresa uzrokovanog lijekovima uobičajenima u domaćinstvu, koji se uzimaju bez recepta (acetilsalicilna kiselina) te biljnih lijekova i tableta (npr. ginko) ili lijekova na recept s antikoagulativnim svojstvima (varfarin). Mnogo manje se zna o međusobnim interakcijama navedenih tvari na razine oksidativnog stresa, osobito u mozgu. U okviru ovog rada štakori soja Y59 tretirani su dnevno s odgovarajućim preporučenim dozama kombinacija acetilsalicilne kiseline, ginka i varfarina, tijekom 15 dana. Razina oksidativnog stresa procijenjena je mjerenjem četiri markera oksidativnog stresa; superoksid dismutaze (SOD), katalaze (CAT), glutationa (GSH) i malondialdehida (MDA) u različitim regijama mozga. Rezultati su pokazali promijenjene razine oksidacijskog stresa u prefrontalnom korteksu, kortikalnoj regiji i malom mozgu izloženih životinja. Ove promjene ukazuju na sinergistički toksični potencijal izazvan simultanim uzimanjem navedenih lijekova.

Abstract (english)

Previous toxicological research showed that blood brain barrier and brain vascular system are highly susceptible to the effects of pharmaceuticals with vasodilatation or anticoagulative properties. The adverse effects on cellular oxidative stress caused by over the counter (OTC) household drugs (acetylsalicylic acid), herbal remedies and pills (for example ginkgo) or prescription drugs (warfarin) are well described. Much less is known about mutual interaction between these substances on oxidative stress levels especially in the brain. Within this study Y59 rats were treated daily with corresponding recommended doses and combinations of acetylsalicylic acid, ginkgo and warfarine during 15 days. Four markers of oxidative stress were assessed; superoxide dismutase (SOD), catalase (CAT), gluthathione (GSH) and malondialdehyde (MDA) in various brain regions. The results have shown changes of oxidative stress levels in prefrontal cortex, cortical regions and cerebellum of exposed animals. These changes implicate synergistic and potentiated toxic potential of combinations of named drugs.

• Keywords: aspirin; varfarin; ginko (Ginkgo biloba); Centella asiatica; oksidativni stres; mozak
• Keywords (english): aspirin; warfarine; ginkgo (Ginkgo biloba); Centella asiatica; oxidative stress; brain
• Language: croatian
• URN:NBN: urn:nbn:hr:217:976398
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-25 08:16:07