Povezanost gestacijskog dijabetesa i metilacije gena za serotoninski prijenosnik u posteljici

master's thesis

• Title (english): Association of gestational diabetes with serotonin transporter gene DNA methylation in placenta of healthy newborns
• Author: Anita Stojanović
• Mentor: Jasminka Štefulj (mentor)
• Mentor: Dubravka Hranilović (sumentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Petra Korać (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-06-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Gestacijski dijabetes je stanje poremećenog metabolizma glukoze koje se javlja tijekom trudnoće kod prethodno zdravih žena. Djeca majki s gestacijskim dijabetesom imaju povećan rizik za razvoj pretilosti u odrasloj dobi. Smatra se da je taj fenomen posljedica razvojnog programiranja pretilosti putem epigenetičkih mehanizama kao što je metilacija DNA. Gen za serotoninski prijenosnik, transmembranski protein odgovoran za unos neurotransmitera serotonina u stanicu, ima važnu ulogu u regulaciji uzimanja hrane i kontroli tjelesne težine te je uključen u etiopatogenezu pretilosti. U ovom radu, postavili smo hipotezu da bi metilacija DNA gena za serotoninski prijenosnik mogla biti uključena u razvojno programiranje pretilosti u gestacijskom dijabetesu, te smo istražili moguću povezanost gestacijskog dijabetesa s razinom metilacije DNA gena za serotoninski prijenosnik u tkivu posteljice zdrave novorođenčadi. Za analizu metilacije DNA koristili smo metodu bisulfitnog sekvenciranja. Razina metilacije gena za serotoninski prijenosnik bila je značajno snižena u trudnoća kompliciranih dijabetesom (N=19) u odnosu na zdrave trudnoće (N=32), dok polimorfizam gena za serotoninski prijenosnik nije imao utjecaj na razinu metilacije. Naši rezultati po prvi put opisuju obrazac metilacije promotorske regije gena za serotoninski prijenosnik u humanoj posteljici, te predstavljaju preliminarni dokaz za njegovu moguću ulogu u razvojnom programiranju u gestacijskom dijabetesu.

Abstract (english)

Gestational diabetes is an altered glucose metabolism state in pregnancy in previously healthy women. Children of mothers with gestational diabetes have an increased risk of obesity as adults. It is considered that this phenomenon is a product of fetal programming through epigenetic mechanisms like DNA methylation. Gene for serotonin transporter, transmembrane protein responsible for serotonin neurotransmitter cell intake has an important role in regulation of food intake, and weight control. It is included in etiopathogenesis of obesity. In this work, we had a hypothesis that DNA methylation of the gene for serotonin transporter could be involved in fetal programming of obesity in gestational diabetes. We investigated the possible relationship of gestational diabetes with the level of DNA methylation of the gene for serotonin transporter in the placental tissue of healthy newborns. For DNA methylation analysis we used bisulphite sequencing. The level of methylation of the gene for serotonin transporter was significantly lower in pregnancies complicated by gestational diabetes (N = 19) compared to pregnancies with no complications (N = 32), while polymorphism of the gene for serotonin transporter had no influence on methylation levels. Our results, for the first time, describe the methylation pattern of the promoter region of the gene for serotonin transporter in the human placenta, and present preliminary evidence for its possible role in developmental programming in gestational diabetes.

• Keywords: gestacijski dijabetes; metilacija DNA; fetalno programiranje; serotoninski prijenosnik
• Keywords (english): gestational diabetes; DNA methylation; fetal programming; serotonin transmitter
• Language: croatian
• URN:NBN: urn:nbn:hr:217:729610
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-25 08:31:26