Usporedba genetičke raznolikosti između i unutar kontinentalnih i primorskih populacija pitomog kestena (Castanea sativa Mill.) u Hrvatskoj

master's thesis

• Title (english): Comparison of genetic diversity of continental and coastal populations of European sweet chestnut (Castanea sativa Mill.) in Croatia
• Author: Matija Dvorski
• Mentor: Zlatko Liber (mentor)
• Committee member: Zlatko Liber (predsjednik povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Tajana Begović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Genetička raznolikost jedan je od najvažnijih elemenata prilagodbe svih živih bića na neprestane promjene u okolišu. Intenzivno iskorištavanje kestenovih šuma i bolest poznata kao rak kestenove kore ostavili su trajne negativne posljedice na populacije europskog pitomog kestena. Svaka spoznaja o genetičkoj varijabilnosti europskog pitomog kestena izravno doprinosi razvoju učinkovitijih planova zaštite i gospodarenja ovom vrstom. Cilj ovog diplomskog rada je bio usporediti genetičku raznolikost između i unutar tri kontinentalne (Bilogora, Medvednica, Moslavačka gora) i tri primorske populacije (Poreč, Buje, Cres) pitomog kestena u Hrvatskoj analizom devet SSR i devet EST-SSR mikrosatelitnih biljega. Dobiveni rezultati nisu potvrdili jasnu genetičku diferencijaciju kontinentalnih i primorskih populacija pitomog kestena u Hrvatskoj. Svojom genetičkom raznolikošću populacije Medvednica, Bilogora, Moslavačka gora i Buje ukazivale su na prirodno podrijetlo, dok su populacije Poreč i Cres ukazivale na jako veliki antropogeni utjecaj u njihovom formiranju. Protok gena iz nasada lovranskih maruna u prirodnu populaciju Cres moguće je objašnjenje specifične genetičke raznolikosti ove populacije, dok bi populacija Poreč mogla biti kombinacija gena talijanskih prirodnih populacija i/ili kultivara, istarskih prirodnih populacija i lovranskog maruna. U odvojenim SSR i EST-SSR analizama svi dobiveni populacijsko-genetički parametri bili su gotovo isti pa se u budućim istraživanjima preporučuje analiza ujedinjenih podataka.

Abstract (english)

Genetic diversity is one of the most important elements in adapting of all living beings to the continual changes in the environment. The intensive exploitation of chestnut forests and the disease known as chestnut blight have left negative effects on the populations of European chestnut. Any knowledge of the genetic variability of the chestnut directly contributes to the development of more effective conservation and management plans for this species. The aim of this thesis was to compare genetic diversity between and within three continental (Bilogora, Medvednica, Moslavačka gora) and three coastal populations (Poreč, Buje, Cres) of European chestnut in Croatia using nine SSR and nine EST-SSR microsatellite markers. The obtained result did not confirm the clear genetic differentiation between continental and coastal populations of European chestnut in Croatia. Due to their genetic diversity, the populations of Medvednica, Bilogora, Moslavačka Gora and Buje indicated their natural origin, while the populations of Poreč and Cres indicated a very large anthropogenic influence during their formation. Gene flow from Lovran Marron plantations to the natural population of Cres is a possible explanation for the specific genetic diversity of this population, while the population Poreč could be a combination of the genes of Italian natural populations and / or cultivars, Istrian natural populations and Lovran Marron. In the separate SSR and EST-SSR analyzes, all obtained population-genetic parameters were almost the same, so the recommendation is that in the future data analysis should be applied on the combined data set.

• Keywords: pitomi kesten; genetička raznolikost; mikrosatelitni biljezi
• Keywords (english): European chestnut; genetic diversity; microsatellite markers
• Language: croatian
• URN:NBN: urn:nbn:hr:217:876036
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-29 10:16:21