Učinak bioaktivnih molekula trnine (Prunus spinosa L.) na lipidnu peroksidaciju i antioksidacijski sustav mozga

master's thesis

• Title (english): The effect of bioactive molecules from blackthorn (Prunus spinosa L.) on lipid peroxidation and antioxidation system of the brain
• Author: Damjan Božić
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Željka Vidaković Cifrek (član povjerenstva)
• Committee member: Damir Sirovina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ekstrakt cvijeta trnine (ECT) bogat je bioaktivnim molekulama – polifenolima. Metodom tekućinske kromatografije ultravisoke djelotvornosti – masene spektrometrije (eng. skraćenica UPLC-MS) otkrivena su 32 polifenolna spoja, najviše iz grupa flavonola, flavan-3-ola i fenolnih kiselina. C57BL/6 miševi su dnevno dozirani sa 25 mg/kg tjelesne težine miša ECT polifenola u periodu od 28 dana. 1., 7., 14., 21. i 28. dana su mjerene maksimalne koncentracije pojedinih polifenola u mozgu te oksidacijski markeri: vrijednosti superoksid dismutaze (SOD), katalaze (CAT), ukupnog glutationa (GSH), malondialdehida (MDA) i karboniliranih proteina (PC). U mozgu mniševa tretiranih ECT detektirano je 11 polifenola statistički značajno povećanih (p≤0,05) u odnosu na kontrolnu skupinu (KO) s ukupnim povećanjem od oko 2,5 puta. Najviše koncentracije pokazuju kvercetin-rhamnozid, kvercetin-3-rutinozid, kvercetin-pentozid, (-)-epigalokatehin-3-galat, te 4-O-kafeoilkvinična kiselina i 4-p-kumaroilkvinična kiselina. Zabilježena su smanjenja (p≤0,05) lipidne peroksidacije (MDA) i oštećenih (karboniliranih) proteina (PC), te povećanja (p≤0,05) koncentracije GSH i enzimske aktivnosti (SOD, CAT) 21. te 28. dan u ECT grupi. Zaključno, ti polifenoli uvelike doprinose antioksidacijskoj zaštiti reducirajući lipidnu peroksidaciju i oksidacijski stres.

Abstract (english)

The extract from the blackthorn flower (ECT) is rich with bioactive molecules – polyphenols. With Ultra-High Performance Liquid-Chromatography Tandem Mass Spectrometry (UPLC-MS) method 32 different polyphenolic compounds were detected, mostly from groups of flavonols, flavan-3-ols and phenolic acids. C57BL/6 mice recived oral daily repeted doses of 25 mg/kg body weight of ECT polyphenols for 28 days. 1st, 7th, 14th, 21st and 28th day were mesured maximum brain concentrations of individual ECT polyphenols and brain oxidative markers; superoxide dismutase (SOD), catalase (CAT), glutathion (GSH), malodialdehyde (MDA) and protein carbonyls (PC). 11 polyphenols detected from the brain were found to be statistically significantly increased (p≤0.05) in ETC group relative to the control group (KO) with a total increase of about 2,5-fold. Compounds with the highest concentrations mesured are: quercetin-rhamnosid, quercetin-3-rutinosid, quercetin-pentosid, (-)-epigallocatechin-3-gallate, 4-O-caffeoylquinic acid and 4-p-coumaroylquinic acid. Lipid peroxidation (MDA) and protein carbonyls (PC) decreases (p≤0,05), together with enzymatic activitys (SOD, CAT) and koncentrations of GSH increases (p≤0,05) were recorded on 21st and 28th day in the ECT group. In conclusion, these polyphenols greatly contribute to antioxidant protection by reducing lipid peroxidation and oxidative stress.

• Keywords: trnina; polifenoli; oksidacijski stres; markeri; MDA; PC
• Keywords (english): blackthorn; polyphenols; oksidative stress; markers; MDA; PC
• Language: croatian
• URN:NBN: urn:nbn:hr:217:746210
• Study programme: Title: Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra edukacije biologije (magistar/magistra edukacije biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-09-27
• Created on: 2019-11-29 10:41:39