Biomineralization in the Sea Hare Aplysia punctata Initiated by Nano-Dolomite

Scientific paper - Original scientific paper

• Author: Anđelka Tonejc
• Author: Davorin Medaković
• Author: Stanko Popović
• Author: Andrej Jaklin
• Author: Mirjana Bijelić
• Author: Ivana Lončarek
• Author's institution: University of Zagreb; Faculty of Science; (Department of Physics)
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Physics

Abstract (english)

X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED), high resolution transmission electron microscopy (HRTEM), environmental scanning electron microscopy (ESEM) and energy dispersive X-ray analysis (EDS) were used in study of starting biomineralization processes in embryos of the sea hare species Aplysia punctata. 10 days old embryos appeared amorphous according to XRD patterns. TEM of the same sample showed that first grains of nanocrystalline dolomite began to form in the amorphous area. The identification of dolomite was confirmed according to TEM dark field images and SAED, as well as by HRTEM. In further development stages of the embryos very faint aragonite rings became visible by SAED. It was shown that the biomineralization process in A. punctata started by formation of the dolomite nanograins which served as centres of crystallization for further aragonite deposition in the larval shell. The creation of unusual intermediate crystalline phase of nano-dolomite in A. punctata embryos is of equal interest for biologist and ecologist as an evolutionary ancestral trait of molluskan biomineralization, as well as for materials scientists, as a promising template in potential bioengineering application and design of appropriate biomimetic routes that could lead to the development of new implantable biomaterials. The discussion of the present results is based on recent knowledge on general biomineralization in mollusks.

• Keywords (english): aplysia punctata; biomineralization; dolomite; X-ray diffraction (XRD); transmission electron microscopy (TEM); environmental scanning electron microscopy (ESEM); energy dispersive X-ray analysis (EDS)
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 87, no. 2, pp. 143-152
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• DOI: https://doi.org/10.5562/cca2403
• URN:NBN: urn:nbn:hr:217:307141
• Publication: 2014
• Project: Number: 098-0982705-2727; Title (croatian): Procesi biomineralizacije morskih organizama; Leader: Davorin Medaković; Jurisdiction: Croatia; Funding stream: ZP
• Project: Number: 119-0982886-1009; Title (croatian): Struktura i svojstva posebnih nanomaterijala dobivenih suvremenim tehnikama; Leader: Antun Tonejc; Jurisdiction: Croatia; Funding stream: ZP
• Document URL: https://hrcak.srce.hr/126968
• Type of resource: Text
• Access conditions: Open access
• Created on: 2019-11-29 15:38:10