Utjecaj nanočestica srebra na vijabilnost i oksidativni stres ljudskih keratinocita

master's thesis

• Title (english): Impact of silver nanoparticles on viability and oxidative stress response in human kerationcytes
• Author: Emerik Galić
• Mentor: Ivan Pavičić (mentor)
• Mentor: Inga Marijanović (mentor)
• Committee member: Inga Marijanović (predsjednik povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Ivana Buj (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-02-08, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Nanočestice srebra (AgNP) jedan su od najčešće korištenih nanomaterijala. Usprkos širokoj primjeni, njihov utjecaj na žive organizme je i dalje predmet istraživanja. U ovom radu je istražen utjecaj triju vrsta nanočestica srebra, stabiliziranih različitim omotačima, na vijabilnost i oksidativni stres ljudskih keratinocita. Pri tome se istražio učinak AgNP u i bez prisustva proteina koji u biološkim sustavima tvore na nanopovršini proteinsku koronu. Proteinska korona može modulirati interakciju nanočestica sa živim sustavima. Rezultati dobiveni primjenom MTS metode na mikrotitarskom čitaču i metode Live/Dead na protočnom citometru pokazuju da su negativno nabijene AgNP citotoksične pri najvišoj testiranoj koncentraciji u uvjetima bez proteinske korone, dok su pozitivno nabijene AgNP pokazale citotoksičnost u prisustvu proteinske korone. Neutralne AgNP nisu pokazale citotoksičnost u ispitivanom koncentracijskom rasponu. Mjerenjem ukupnog sadržaja slobodnih radikala i glutationa (GSH) nije dokazan oksidativni stres u ljudskih keratinocita nakon tretmana nanočesticama srebra.

Abstract (english)

Silver nanoparticles (AgNP) are one of the most widely used types of nanomaterials. However, their effects on living organisms is still the subject of ongoing research. In this thesis, cell viability and oxidative stress response of human keratinocytes exposed to three different types of AgNP were investigated. In addition, cell response was evaluated after addition of proteins in test media to determine the effect of protein corona formed on AgNP surface. Recent studies suggest that protein corona may modulate the biological effect of nanoparticles. Testings were performed by MTS assay and Live/Dead flow cytometry tests. Obtained results demonstrated that negatively charged AgNP were cytotoxic to keratinocytes only at the highest concentrations applied without the presence of protein corona, while positively charged AgNP showed cytoxicity at the highest tested concentrations after protein corona formation. Neutral AgNP were not cytotoxic in the tested concentration range. Oxidative stress response in cells was tested by mesuring total amount of peroxy radicals and glutation. Results did not indicate any oxidative stress in human keratinocytes exposed to tested concentrations of AgNP.

• Keywords: Nanočestice srebra; ljudski keratinociti; vijabilnost; citotoksičnost; oksidativni stres; proteinska korona
• Keywords (english): silver nanoparticles; human keratinocytes; viability; cytotoxicity; oxidative stress; protein corona
• Language: croatian
• URN:NBN: urn:nbn:hr:217:717653
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-08
• Created on: 2019-12-04 09:29:46