Promjene u ekspresiji epitopa proteina GFAP i PSA-NCAM nakon primjene kuprizona u miševa C57Bl/6

master's thesis

• Title (english): Changes in expression of GFAP and PSA-NCAM epitopes after application of demyelinating substance cuprizone in mouse strain C57black/6
• Author: Ada Zelen
• Mentor: Marija Heffer (mentor)
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Tretiranjem miševa kuprizonom dolazi do masovne apoptoze te demijelinizacije neurona. Demijelinizaciju, uz propadanje oligodendrocita, prate pojava astroglije i mikroglije. Tijekom procesa demijelinizacije na aksolemi dolazi do ekspresije epitopa PSA-NCAM koji djeluje kao negativni regulator mijelinizacije, spriječavajući vezanje mijelin-stvarajućih stanica (oligodendrocita) na akson neurona. PSA-NCAM ujedino je i marker matičnih stanica i migracije. Demijelinizaciju, uz propadanje matičnih stanica prati i pojava astroglioze (porast broja reaktivnih astrocita). Astrociti mijenjaju morfologiju i ekspresiju proteina, između ostalog i proteina GFAP koji je klasični imunohistokemijski marker reaktivnih astrocita. Cilj ovog istraživanja bio je procijeniti obujam neurogeneze i gliogeneze do koje dolazi nakon primjene bakrenog kelatora kuprizona. Istraživanje je provedeno na miševima soja C57black/6 starosti 3 mjeseca, koji su hranjeni kuprizonom u trajanju od devet tjedana. Procjena demijelinizacijskog oštećenja i regeneracijskog kapaciteta načinjena je imunohistokemijskim označavanjem protutijelima GFAP i PSA-NCAM u regiji somatosenzornog korteksa. Iz ovog istraživanja možemo zaključiti da djelovanjem kuprizona dolazi do demijelinizacije neurona koja je praćena velikim izražajem PSANCAM epitopa i astroglioze.

Abstract (english)

Treatment of a mice with cuprizone leads to massive apoptosis and demyelination of neurons. Demyelination with declined number of oligodendrocytes is also accompanied by the emergence of astroglia and microglia. During the process of demyelination surface of axolema is expressing PSA-NCAM epitopes which act as a negative regulator of myelination, preventing the binding of myelin creating cells (oligodendrocytes) on axons of neurons. PSA-NCAM also is a marker of stem cells and migration. Demyelination, with a decadence of stem cells follows the appearance of astrogliosis (increased number of reactive astrocytes). Astrocytes change their morphology and expression of proteins, including GFAP, commonly used immunohistochemical marker of reactive astrocytes. The aim of this study was to estimate the scope of neurogenesis and gliogeneze that occurs after applying a copper chelator cuprizone. The study was conducted on mice strains C57black / 6, age 3 months, who were fed with cuprizone for a period of nine weeks. Valuation of damage and regeneration capacity was made by immunohistochemical labeling with antibodies GFAP and PSA-NCAM in the region of the somatosensory cortex. From this study it can be concluded that the cuprizone leads to demyelination of neurons and that demyelination is accompanied by overexpression of PSA-NCAM epitopes and astrogliosis.

• Keywords: neurotoksin; demijelinizacija; remijelinizacija; neurogeneza; gliogeneza
• Keywords (english): neurotoxin; demyelination; remyelination; neurogenesis; gliogenesis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:236832
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-25 10:04:40