Antioksidativni učinci kavene kiseline: mogućnost primjene u dijabetesu

master's thesis

• Title (english): Antioxidant activities of caffeic acid: applicability in diabetes
• Author: Dijana Bonić
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Dijabetes mellitus je bolest s visokom stopom rasta. Cilj ovog istraživanja bio je proučiti moguće zaštitne učinke kavene kiseline na nastala oštećenja u miševa s dijabetesom prouzročenim aloksanom. Tip 1 dijabetes induciran je u miševa davanjem aloksana u dozi od 75 mg kg-1 iv. Nakon 9. dana od iniciranja aloksanom dolazi do gubitka tjelesne mase te povećanja glukoze, triglicerida i ukupnog kolesterola u serumu u odnosu na normalnu kontrolnu skupinu. Kavena kiselina (50 mg kg-1) injicirana miševima s dijabetesom tijekom sedam dana bila je učinkovita u održavanju normalne tjelesne mase životinja, smanjenju % hemolize eritrocita, koncentracije triglicerida i kolesterola u krvi oboljelih miševa, te povećanju funkcionalne aktivnosti makrofaga peritonealne šupljine ali nije pokazala učinak na preživljenje miševa. Rezultati komet testa ukazuju da je kavena kiselina smanjila oštećenja DNA u limfocitima periferne krvi uzrokovana oksidativnim stresom te da kafeinska kiselina ima obećavajući učinak na smanjenje negativnih posljedica razvoja dijabetesa, čemu doprinosi njen antioksidativni potencijal.

Abstract (english)

Diabetes mellitus is a disease with high growth rate. The present study was designed to investigate the possible antidiabetic and protective effect of caffeic acid against alloxan-induced oxidative damage in diabetic mice. Type 1 diabetes was induced in mice by injection alloxan in dose of 75 mg kg-1 bwt iv. After 9 days of alloxan injection, there was an apparent reduction in the animal body weight and significant increase in serum glucose, triglyceride and total cholesterol levels as compared to the control healty mice. Treatment of diabetic mice with caffeic acid in dose of 50 mg kg-1 for seven days reduced the decline in body mass,% hemolysis of erythrocytes, blood triglyceride and cholesterol levels and increased functional activity of macrophages peritoneal cavity but did not increase the survival of mice. Additionally comet assay results show that treatment diabetic animals with caffeic acid reduces the alloxan-induced DNA damage to lymphocytes. The results indicate that caffeic acid has promising effects in reducing the negative consequences of developing diabetes, which contributes to its antioxidant potential.

• Keywords: dijabetes; oksidativni stres; aloksan; kavena kiselina; komet test; miš
• Keywords (english): diabetes; oxidative stress; aloxan; caffeic acid; comet assay; mices
• Language: croatian
• URN:NBN: urn:nbn:hr:217:718285
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-25 12:24:04