DNA barkodiranje kao metoda u taksonomskoj identifikaciji biljnih i životinjskih vrsta

master's thesis

• Title (english): DNA barcoding as a method in taxonomical identification of plant and animal species
• Author: Jakob Ćosić
• Mentor: Damjan Franjević (mentor)
• Committee member: Damjan Franjević (predsjednik povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Committee member: Petra Korać (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014-11-20, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

DNA barkodiranje je novo predložena metoda molekularne taksonomije koja korištenjem kratkih univerzalnih sekvenci koje su dovoljno različite kod svih organizama od interesa omogućava brzu i preciznu identifikaciju biološkog materijala. U ovom radu sam istražio uspješnost DNA barkodiranja kao taksonomske metode za identifikaciju biljnih i životinjskih vrsta te barkodirao devet autohtonih hrvatskih biljnih vrsta i divokozu Rupicapra rupicapra L. (Bovidae) te na osnovi dobivenih barkodova rekonstruirao filogenetski odnos populacija divokoze iz Hrvatske. Na kraju sam se dotakao i problema s kojima se ova metoda suočava, najčešće u vidu malih razlika među barkodovima raziličitih vrsta, problematičnog barkodiranja biljaka zbog spore evolucije plastidnog genoma i izolacije biljnih gena i problema pri određivanju životinjskih vrsta zbog specifičnog načina evolucije životinjskog mitohondrijskog genoma te prijedloga za njezino poboljšanje.

Abstract (english)

DNA barcoding is a newly proposed method of molecular taxonomy, which by the usage of short, universal sequences that are divergent enough in all organisms of interest, allows fast and precise identification of biological material. In this paper, I examined the fidelity of DNA barcoding as a taxonomic method for identification of plant and animal species. I also barcoded nine autochthonous Croatian plant species and chamois Rupicapra rupicapra L. (Bovidae), and conducted analysis of phylogenetic relations of chamois populations from Croatia based on collected barcode sequences. In the end, I considered the problems concerning the method, as well as suggestions for its improvement. Considered problems are mostly small differences between barcodes of different organisms, problematic barcoding of plants due to slow evolution of plastid genome and isolation of plant genes and problematic determination of animals due to specific evolution of animal mitochondrial genome.

• Keywords: molekularna taksonomija; matK; rbcL; COI; divokoza
• Keywords (english): molecular taxonomy; matK; rbcL; COI; chamois
• Language: croatian
• URN:NBN: urn:nbn:hr:217:832636
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-26 08:52:03