Utjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša

master's thesis

• Title (english): Effect of Trnp1 orthologs on proliferation of E14 mouse cortical primary culture
• Author: Nika Foglar
• Mentor: Miriam Esgleas (mentor)
• Mentor: Inga Marijanović (sumentor)
• Committee member: Inga Marijanović (predsjednik povjerenstva)
• Committee member: Martina Šeruga Musić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Through the evolution of mammals, the brain size has increased by folding the neocortex. The cause for differences in folding between species could be different regulation of some genes. Trnp1 gene overexpression causes self-renewal of neuronal stem cells in vitro, while in vivo knockdown leads to folding of the cortex in mice. Rate of evolutionary mutation was calculated for Trnp1 orthologs, and correlation between these rates and degree of gyrification was noticed. To determine if evolutionary mutations of Trnp1 have different effect on the self-renewal capacity of cortical primary culture cells, six Trnp1 orthologs were overexpressed in these cells. The six species were Mus musculus, Otolemur garnettii, Macaca mulatta, Mustela putorius, Homo sapiens and Tursiops truncates. Analysis showed that all Trnp1 orthologs increase proliferation, but the effect of each ortholog was similar. Therefore, it is unlikely that evolutionary mutations of Trnp1 caused the differences in folding between these species.

Abstract (croatian)

Kroz evoluciju sisavaca, veličina mozga se povećavala stvaranjem nabora u mozgu. Uzrok različite naboranosti mozga između sisavaca mogao bi biti različita regulacija nekih gena. Prekomjerna ekspresija gena Trnp1 uzrokuje samoobnavljanje živčanih matičnih stanica in vitro, dok in vivo smanjenje ekspresije uzrokuje stvaranje nabora u korteksu miša. Brzina evolucijskih mutacija je izračunata za Trnp1 ortologe te je primijećena korelacija između te brzine i stupnja naboranosti mozga. Kako bi se odredilo imaju li evolucijske mutacije gena Trnp1 različiti efekt na sposobnost samoobnavljanja stanica primarne kulture korteksa, šest ortologa Trnp1 je eksprimirano u stanicama. Odabrane vrste su bile Mus musculus, Otolemur garnettii, Macaca mulatta, Mustela putorius, Homo sapiens and Tursiops truncates. Analiza je pokazala da svi ortolozi Trnp1 povećavaju sposobnost proliferacije, ali su ortolozi imali podjednak učinak. Stoga je malo vjerojatno da su evolucijske mutacije gena Trnp1 uzrokovale razlike u stupnju naboranosti mozga između tih vrsta.

• Keywords: Trnp1 gene cloning; primary cell culture; transfection; immunohistochemistry
• Keywords (croatian): kloniranje gena Trnp1; primarna kultura stanica; transfekcija; imunohistokemija
• Language: english
• URN:NBN: urn:nbn:hr:217:667753
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-02-27
• Created on: 2020-06-09 10:14:39