Utjecaj mješavine antidepresiva i amfetamina na fiziološke pokazatelje i ponašanje invazivne strane vrste signalnog raka Pacifastacus leniusculus (Dana, 1852)

master's thesis

• Author: Katarina Buhin
• Mentor: Sandra Hudina (mentor)
• Mentor: Sandra Radić Brkanac (mentor)
• Committee member: Sandra Hudina (predsjednik povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Vodeni ekosustavi kontinuirano su ugroženi ispuštanjem sve većih količina tzv. „emerging contaminants“ koji uključuju farmaceutike i proizvode za osobnu njegu. Farmaceutici se u okolišu ne pojavljuju kao individualni spojevi, već kao njihove mješavine. Cilj istraživanja bio je ispitati ima li mješavina tri odabrana prioritetna spoja (antidepresiva citaloprama i nortriptilina te opojne droge amfetamina) utjecaj na fiziologiju i ponašanje najuspješnije invazivne strane vrste europskih slatkovodnih ekosustava – signalnog raka (Pacifastacus leniusculus). Rakovi su mješavini spojeva u okolišno relevantnoj koncentraciji bili izloženi sedam dana. U dva navrata – nakon 24 h i nakon sedam dana izlaganja mjereni su fiziološki pokazatelji: koncentracija serotonina u cerebralnim ganglijima, koncentracija glukoze u hemolimfi te koncentracija malondialdehida (MDA) kao biomarkera oksidativnog stresa, te ponašanje: aktivnost u novom prostoru i antagonističke interakcije rakova. Koncentracija serotonina, razina glukoze u hemolimfi, aktivnost i dinamika antagonističkih interakcija nisu se značajno razlikovale između izloženih jedinki i kontrola, no jedinke izložene mješavini spojeva imale su značajno povećanu koncentraciju MDA što pokazuje da mješavina spojeva može uzrokovati oksidativni stres kod ne-ciljanih organizama čak i u okolišno relevantnim koncentracijama. Buduća istraživanja trebalo bi proširiti na utjecaj mješavine spojeva na rast i reprodukciju rakova te produžiti trajanje izlaganja.

Abstract (english)

Aquatic ecosystems are continuously threatened by the release of increasing amounts of “emerging contaminants”, including pharmaceuticals and personal care products. In the environment, pharmaceuticals are present in mixtures but their effects on non-target organisms are largely unexplored. This study aims to examine whether the mixture of three chosen substances (antidepressant citalopram and nortriptyline and illicit drug amphetamine) influences the behavior and physiology of the most successful crustacean invader of European freshwaters - the signal crayfish (Pacifastacus leniusculus). Crayfish were exposed to environmentally relevant concentrations of mixtures for seven days. We measured the effects on physiology (indicators: serotonin concentration in the brain, glucose level in the hemolymph and concentration of malondialdehyde (MDA) as a biomarker of oxidative stress), and behaviour (parameters: activity and antagonistic interactions) after 24 h and after seven days. There was no significant difference in serotonin and glucose concentrations nor in activity or dynamics of antagonistic interactions between exposed individuals and controls. Exposed individuals had significantly increased MDA concentrations which shows that compound mixture can cause oxidative stress in the non-target organisms even in environmentally relevant concentrations. Future research should include the effect of compound mixtures on growth and reproduction and extend the duration of exposure.

• Keywords: farmaceutici; mješavine; signalni rak; serotonin; antagonističke interakcije
• Keywords (english): pharmaceuticals; mixtures; signal crayfish; serotonin; antagonistic interactions
• Language: croatian
• URN:NBN: urn:nbn:hr:217:404184
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-27
• Created on: 2020-07-13 08:40:26