Perzistencija koliformnih bakterija u izvorskoj vodi Papuka

master's thesis

• Title (english): Persistence of coliform bacteria in Papuk spring waters
• Author: Doris Iličić
• Mentor: Goran Palijan (mentor)
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Ivana Buj (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Koliformne bakterije uobičajeni su mikrobiološki pokazatelji kvalitete vode za piće. S obzirom na njihovo fekalno podrijetlo, jednom unesene u okoliš, mogu predstavljati rizik za ljudsko zdravlje. Cilj ovog istraživanja bio je odrediti kakvoću vode, odnosno, odrediti prisutnost ukupnih heterotrofnih te ukupnih i fekalnih koliformnih bakterija u uzorku izvorske vode s četiri odabrana izvora na području Papuka: Jankovac, Kokočak (Vučenović), Žervanjska i Muška voda. Kako bi se simuliralo zagađenje izvorske vode i sedimenta fekalnim materijalom, a s ciljem utvrđivanja sposobnosti perzistencije koliforma, upotrijebljeni su mikrokozmosi koji su u tu svrhu prethodno inokulirani laboratorijski čistom kulturom koliformne bakterije. Rezultati istraživanja pokazali su kako, s obzirom na povećan broj ukupnih heterotrofnih te ukupnih i fekalnih koliformnih bakterija, voda s uzorkovanih izvora nije prikladna za piće. Inokulirani koliformi uspješno su preživjeli sedmodnevnu inkubaciju u svim uzorcima izvorske vode osim u vodi s izvora Jankovac. Stoga se može zaključiti da Jankovac ima, uz utvrđenu nisku sposobnost formiranja biofilma bakterija s ovoga izvora, najmanju mogućnost perzistencije koliforma u slučaju zagađenja izvora. Također, s obzirom da ovi rezultati pružaju samo dio uvida u kakvoću izvorske vode potrebno je provesti dodatne analize, uz kontinuirano praćenje mikrobioloških parametara, kako bismo sa sigurnošću mogli utvrditi zdravstvenu ispravnost iste.

Abstract (english)

Coliform bacteria are most commonly used as microbiological indicators of drinking water quality. Given their faecal origin, once introduced into the environment, they can pose a risk to human health. The aim of this study was to determine water quality by determining the presence of total heterotrophic and total and fecal coliform bacteria in four selected springs in the Papuk area: Jankovac, Kokočak (Vučenović), Žervanjska and Muška voda. In order to simulate the contamination of spring water and sediment with fecal material, microcosms were used. They were previously inoculated with a laboratory-pure culture of coliform bacteria. In the same manner, microcosms were used in order to determine the persistence of inoculated coliforms. The results of the research showed that, given the increased number of total heterotrophic and total and fecal coliform bacteria, water from sampled springs is not suitable for drinking. On the other hand, inoculated coliforms successfully persisted in all spring water samples except in the water from the spring Jankovac. Therefore, it can be concluded that Jankovac has, with the established low ability of bacteria from this spring to form a biofilm, the lowest possibility of coliform persistence in case of pollution of the spring. Also, given that these results provide only a part of the insight into the quality of spring water, it is necessary to carry out additional analyses, with continuous monitoring of microbiological parameters.

• Keywords: voda; koliformi; perzistencija; biofilm; Papuk
• Keywords (english): water; coliforms; persistence; biofilm; Papuk
• Language: croatian
• URN:NBN: urn:nbn:hr:217:223488
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-09-14 08:06:58