Upotreba testova otvorenog polja (open field) i novog objekta (novel object) u neuroznanosti

undergraduate thesis

• Title (english): Open field and novel object tests appliance in neuroscience
• Author: Neven Marković
• Mentor: Duje Lisičić (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Bihevioralna znanost, kao podgrana neuroznanosti, bavi se proučavanjem kognitivnih procesa te obrazaca ponašanja životinja u laboratorijski kontroliranim uvjetima. Test otvorenog polja (open field test – skraćeno OFT) je jedan od najčešće korištenih metoda istraživanja u bihevioralnoj znanosti (Belzung 2014). Uz njega, u zadnje vrijeme test novog objekta (novel object test – skraćeno NOT) dobiva na sve većoj popularnosti. OFT i NOT primjenjivani su na cijelom spektru životinja od beskralježnjaka pa sve do odvedenijih sisavaca poput majmuna. U ovom radu dan je pregled u varijabilnost čimbenika unutar metodologije koji se koriste pri eksperimentalnom istraživanju. Vrlo je široka primjena testova OFT-a i NOT-a u medicini gdje se može pratiti djelovanje anksiolitika, koje su posljedice lezija mozga ili genetskih mana, otkriti sedacija ili hiperaktivno ponašanje, kao i napraviti procjenu učenja i pamćenja te istražiti općeniti učinak lijekova i supstanci na ponašanje (Belzung 2014). Mnogi znanstvenici iznijeli su kritiku na OFT i NOT gdje se iznosi problematika nekoordiniranosti u metodologiji odnosno samom procesu provedbe eksperimenta. Čimbenici poput rukovanja životinja prije testiranja, oblik novog objekta koji se koristi u NOT-u, odvajanje životinje od socijalne grupe pri testiranju te prisilno sučeljavanje organizma s OF-om uvelike utječu na daljnje ponašanje životinje u eksperimentu. Stoga je bitno svaki korak specificirati te zajedničkim naporima doći do rješenja kako smanjiti utjecaj tih faktora.

Abstract (english)

Behavioural science, as a neuroscience branch, studies cognitive processes and patterns of animal behaviour in laboratory-controlled conditions. The OFT (abbreviation for open field test) is one of the most commonly used research methods in behavioural science (Belzung 2014). Along with it, the NOT (abbreviation for novel object test) has been gaining in popularity lately. OFT and NOT have been applied to the broad-spectrum of animals from invertebrates to advanced mammals such as monkeys. This paper provides an overview of factors variability within the methodology used in experimental research. OFT and NOT are extensively used in medicine where they reveal the effects of anxiolytics, explore which are the consequences of brain lesions and genetic defects, detect sedation or ADHD behaviour, as well as assess learning and memory and reveal effect of drugs and substances (Belzung 2014). Many scientists have criticised OFT and NOT about the lack of coordination in the methodology and about the process of conducting the experiment issue. Factors such as handling animals prior testing, the shape of the novel object used in the NOT, separation of the animal from the social group for testing and forced confrontation of the organism with OF greatly influence the further behaviour of the animal in the experiment. Hence, it is important to specify all the steps used in research and come up with a solution to reduce the impact of these factors.

• Keywords: neuroznanost
• Language: croatian
• URN:NBN: urn:nbn:hr:217:617371
• Study programme: Title: Environmental Sciences; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) struke znanosti o okolišu (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-09-25 08:55:01