Genska ekspresija i filogenija MATE (Multidrug and toxin extrusion) proteina u zebrici, Danio rerio (Hamilton, 1822)

master's thesis

• Title (english): Expression profiling and phylogeny of MATE (Multidrug and toxin extrusion) proteins in zebrafish, Danio rerio (Hamilton, 1822.)
• Author: Petra Krznar
• Mentor: Tvrtko Smital (mentor)
• Mentor: Goran Klobučar (mentor)
• Committee member: Goran Klobučar (predsjednik povjerenstva)
• Committee member: Tvrtko Smital (član povjerenstva)
• Committee member: Kristian Vlahoviček (član povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-02-11, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Apsorpcija, distribucija, transformacija i eliminacija predstavljaju ključne dijelove metabolizma endo- i ksenobiotika. U posljednjem koraku eliminacije sudjeluju i tzv. MATE (Multidrug and toxin extrusion) proteini koji izbacuju kemikalije iz stanice. MATE proteini su pronađeni u gotovo svim organizmima, od bakterija do ljudi. Kod ljudi su pronađena tri MATE proteina (MATE1, MATE2, MATE2-K), dok je kod većine kralježnjaka pronađeno dva MATE proteina. MATE geni su prisutni u svim tkivima, pri čemu su najviše eksprimirani u bubrezima i jetri, najvažnijim detoksikacijskim organima. Trenutno znanje o MATE proteinima je bazirano isključivo na MATE proteinima sisavaca. Stoga smo željeli istražiti MATE proteine riba, koristeći zebricu (Danio rerio, Hamilton, 1822.) kao modelni organizam. Prikazali smo prvu komparativnu filogeniju MATE proteina od bakterija do ljudi i identificirali pet MATE gena u zebrici. MATE geni u zebrici su dodatno karakterizirani određivanjem ekspresije mRNA u tkivima muških i ženskih riba.

Abstract (english)

Absorption, distribution, transformation and elimination present key processes of metabolism of endo- and xenobiotics. The last step of their elimination includes so-called MATE (Multidrug and toxin extrusion) proteins which efflux chemicals from the cell. MATE proteins are found in almost all organisms, ranging from bacteria to humans. Three MATE proteins are found in humans (MATE1, MATE2, MATE2-K), while in most vertebrates two MATEs are found. MATE genes are present ubiquitously throughout body and are mostly expressed in kidneys and liver, the most important detoxification organs. Since current knowledge about MATEs is limited to mammals, the idea behind this study was to explore MATEs in fish using zebrafish (Danio rerio, Hamilton, 1822) as a model species. First comprehensive phylogenetic analysis of MATEs, from bacteria to humans have been conducted and five MATE genes have been identified in zebrafish. Five zebrafish MATEs where further characterized in terms of their mRNA expression across tissues of male and female fish.

• Keywords: ADME; izbacivanje iz stanice; qRT-PCR; genomska duplikacija; topologija; klasifikacija
• Keywords (english): ADME; efflux; qRT-PCR; genome duplication; topology; classification
• Language: croatian
• URN:NBN: urn:nbn:hr:217:746474
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 09:53:51