Učinkovitost krizina u supresiji psorijaznih lezija kože miša prouzročenih iritantom di-n-propil disulfidom

master's thesis

• Title (english): Effectivity of chrysin in the supression of psoriatic lesion on mouse skin irritated with di-n-propyl disulfide (PPD)
• Author: Maja Mataković
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-01-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Reaktivni kisikovi radikali imaju svoju ulogu u normalnoj i u abnormalnoj fiziologiji kože. Znanstvena istraživanja pokazala su ulogu reaktivnih kisikovih radikala u patogenezi psorijaze te učinkovitost antioksidativne terapije u liječenju različitih kožnih oboljenja poput psorijaze, ali i ostalih poremećaja gdje oksidativni stres ima patogenu ulogu. Obzirom da je krizin flavonoid s antioksidativnim i protuupalnim značajkama, istražili smo učinak krizina na psorijazno kožno oboljenje miša, uzrokovano di-n-propil disulfidom, različitim metodama kao što su: određivanje broja stanica u peritonealnoj šupljini, mikronukleus-test, komet-test, termografsko snimanje, određivanje koncentracije MDA i GSH, određivanje funkcionalne aktivnosti makrofaga u peritonealnoj tekućini i patohistološka analiza promjena na koži pokusnih životinja. Temeljem rezultata zaključujemo da krizin pokazuje protuupalni učinak, te da bi njegova primjena mogla poslužiti u sprječavanju i liječenju upalnih promjena psorijaze, bez toksičnih posljedica.

Abstract (english)

Reactive oxygen species play a role in a normal and abnormal dermal physiology. Scientific studies have shown that reactive oxygen species play a major role in the pathogenesis of psoriasis and that compounds inducing effective antioxidant responses are very effective in the treatment of various skin diseases, including psoriasis and other disorders where oxidative stress plays a pathogenic role. Chrysin is a flavonoid that proved to be very effective in antioxidant and anti-inflammatory activity. Therefore in these experiments we determined the effect of chrysin on mouse psoriatic skin condition caused by di-n-propyl disulfide. We used different methods such as the determination of the number of cells in the abdominal cavity, micronucleus test, comet assay, thermographic imaging, determination of the functional activity of macrophages in the peritoneal fluid, histopathological analysis of changes in the skin of experimental animals, and the determination of parameters of oxidative stress. Our research has shown that anti-inflammatory effects of chrysin could be used in the prevention and treatment of psoriatic skin lesions without genotoxic effects.

• Keywords: psorijaza; oksidativni stres; lipidna peroksidacija; glutation; histopatološka analiza
• Keywords (english): psoriasis; oxidative stress; lipid preoxidation; glutathione; hystopathologic analysis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:163046
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 10:24:12