Fetalni obrazac ekspresije neurokana u neostrijatumu čovjeka

master's thesis

• Title (english): Expression pattern of neurocan in fetal human neostriatum
• Author: Martina Mavrović
• Mentor: Nataša Jovanov Milošević (mentor)
• Mentor: Dubravka Hranilović (sumentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Nataša Jovanov Milošević (član povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Zoran Tadić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-02-12, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ovaj rad opisuje izražaj (ekspresiju) hondroitin-sulfat proteoglikana neurokana (NCAN) u neostrijatumu fetusa čovjeka od 12. do 40. tjedna gestacije (TG), primjenom indirektne imunohistokemijske metode na postmortalnom moždanom tkivu. Utvrđen je vremenski i prostorni obrazac izražaja neurokana u fetalnom neostrijatumu. Neurokan je eksprimiran u strijatumu od 16. do 25. TG. U ranoj fazi ekspresije 16.-18. TG neurokan je prisutan u staničnim otočićima – striosomima, te sa 19./20. TG pokazuje pomak izražaja iz striosoma u matriks. Nakon 23. TG intenzitet izražaja neurokana u fetalnom neostrijatumu opada do 27. TG kada prestaje njegova ekspresija. Najveći intenzitet izražaja neurokana između 19. i 24. TG pokazuje usko područje bez stanica koje obrubljuje striosome - peristriosomi ili perimetri, koji su najbolje vidljivi tijekom fetalnog i ranog posnatalnog razvoja. Iz ovog istraživanja možemo zaključiti da selektivna ekspresija neurokana u odjeljcima fetalnog strijatuma odražava razlike u vremenu uraštanja i sazrijevanju aferenata u strijatalne odjeljke, te da neurokan ima važnu ulogu u formiranju granica između strijatalnih odjeljaka.

Abstract (english)

The aim of this study is to evaluate the expression pattern at histological level of the chondroiotin-sulphate proteoglycan neurocan (NCAN) in the human fetal neostriatum from gestation weeks (GW) 12 to 40, using indirect immunohystochemical staining on postmortal brain tissue. Neurocan is expressed in the neostriatum from 16 – 25 GW. In the early phase of expression, from 16 – 18 GW, neurocan is expressed in the cell islands – striosomes, and at 19/20 GW shows a shift in expression from the striosomes to the surrounding matrix. After the 23rd GW neurocan expression declines untill GW 27 when its expression ceases. Neurocan shows the highest rate of expression between the 19th - 23 GW in a tight cell-free zone that surrounds the striosomes – the peristriosomes or perimeters, which are most pronounced during fetal and early postnatal development. We propose that the selective expression of neurocan in the different modules of the fetal striatum reflects the differences in the sequence of growth and maturation of striatal afferents, and/or that neurocan has a role in forming the boundaries between striatal modules.

• Keywords: fetalni razvoj; hondrotin-sulfat proteoglikani; bazalni gangliji; striosomi
• Keywords (english): fetal development; chondroiotin-sulphate proteoglycans; basal ganglia; striosomes
• Language: croatian
• URN:NBN: urn:nbn:hr:217:032177
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 10:37:16