Prisutnost mikroplastike i histološka analiza probavne žlijezde dagnji Mytilus galloprovincialis Lamarck, 1819 s područja sjevernog Jadrana

master's thesis

• Title (english): Microplastics and histological analysis of the digestive gland of the mussels Mytilus galloprovincialis Lamarck, 1819 from the northern Adriatic
• Author: Karla Štefanko
• Mentor: Ines Kovačić (mentor)
• Mentor: Petar Kružić (sumentor)
• Committee member: Petar Kružić (predsjednik povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-12-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Zbog nepravilnog odlaganja otpada, nedovoljnog gospodarenja otpadom i otpadnim vodama dolazi
doznačajnog nakupljanja čestica mikroplastike u kopnenim, slatkovodnim i morskim
ekosustavima.Ulaskom u ekosustave mikroplastika može imati znatni utjecaj na morske organizme, a
posebice školjkaše. Cilj ovog rada je utvrditi broj, oblik i veličinu čestica mikroplastike u probavnoj
žlijezdi dagnje Mytilus gallopovincialis uzorkovanena 6 postaja u sjevernom Jadranu (područja uz
gradove Pula, Rovinj i Kopar). Provedena je histomorfometrijska analiza i semikvantitativna procjena
atrofije mikrotubula probavne žlijezde dagnji. Najveći broj identificiranih čestica mikroplastike pronađen
je u probavnim žlijezdama dagnji uzorkovanih na postaji Rovinj (30.2 ±29.9), a najmanje na postaji
Katarina (8 ±2.4) i Strunjan (10 ± 0.5). Najzastupljenije čestice mikroplastike prema boji bile su bijele
boje (91%), a najmanje zastupljene žute boje (1%).Prema veličini, najzastupljenije su bile čestice 5 –
10μm, a prema obliku film.Histološka analiza probavne žlijezde dagnji pokazala je da najveći utjecaj
mikroplastika može imati na atrofiju probavnih tubula i smanjenje debljine epitela probavnih kanalića
(Rovinj, Kopar). Atrofija probavnih tubula i smanjenje debljine probavnih kanalića pokazali su se ka

Abstract (english)

Due to improper waste disposal, insufficient waste and wastewater management, microplastic
particles significantly accumulate in terrestrial, freshwater and marine ecosystems. By in entering
ecosystems, microplastics can have a significant impact on marine organisms, especially shellfish.
The aim of this study was to determine the number, shape and particle size of microplastics in the
digestive gland of mussel Mytilus gallopovincialis collected at 6 stations in the northern Adriatic (areas
near the cities of Pula, Rovinj and Koper). Histomorphometric analysis and semiquantitative atrophy of
the digestive gland microtubules were performed. The largest number of identified microplastic
particles was found in the digestive glands of mussels sampled at the Rovinj station (30.2 ± 29.9), and
the least at the Katarina (8 ± 2.4) and Strunjan (10 ± 0.5) stations. The most common microplastic
particles by color were white (91%), and the least common were yellow (1%). According to the size,
the smallest particles from 5 to 10μm the most represented, and according to the shape, the film was
the most abundant. Histological analysis of the digestive gland of mussels showed that microplastics
can have the greatest impact on the atrophy of the digestive tubules and a decrease in the thickness
of the digestive gland epithelium (Rovinj, Kopar). The atrophy of the digestive tubules and decrease in
the thickness of the digestive gland epithelium have been shown to be a useful tool for monitoring the
influence od microplastics on changes in the histology of the digestive gland.

• Keywords: mikroplastika; mediteranska dagnja Mytilus galloprovincialis; histološke analize
• Keywords (english): micoplastic particles; Mediterranean mussel; Mytilus gallopovincialis; histological analysis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:321022
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-01-28 10:50:41