Vrijednosti kalprotektina i predviđanje tijeka juvenilnog spondiloartritisa

master's thesis

• Title (english): Calprotectin values and the course of juvenile spondyloarthropathies
• Author: Kim Naletilić
• Mentor: Miroslav Harjaček (mentor)
• Mentor: Ana Galov (mentor)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Miroslav Harjaček (član povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Zoran Tadić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Juvenilni spondiloartritis čini skupinu kroničnih upalnih bolesti kod kojih su zahvaćeni periferni zglobovi, mjesto gdje se tetive vežu uz kost (enteze), ali i zglobovi zdjelice i kralježnice. Spondiloartropatije učestalo su povezane s kroničnim upalnim bolestima crijeva. Kalprotektin, protein kojim obiluju neutrofilni granulociti, jedan je važnih proteina upalnog odgovora i obrane od patogena. Istraživanja su pokazala kako je fekalni kalprotektin obećavajući biljeg za praćenje aktivnosti upalnih bolesti crijeva, ali i uspješnosti terapije. Cilj ovog rada je utvrditi može li kalprotektin služiti za predviđanje ili praćenje tijeka bolesti kod bolesnika s juvenilnim spondiloartritisom. U tu svrhu korištene su dvije metode za detekciju kalprotektina u stolici, poliklonska ELISA Calprest kompletom i monoklonska EliA Calprotectin automatizirana metoda. Rezultati pokazuju višu razinu korelacije mjerenja dobivenih prvom metodom s drugim parametrima upale (brzina sedimentacije eritrocita i C-reaktivni protein). Ipak, samo na temelju ovih rezultata fekalni kalprotektin nije moguće koristiti kao adekvatnu mjeru praćenja tijeka juvenilnog spondiloartritisa.

Abstract (english)

Juvenile spondyloarthropathies are a group of chronic inflammatory disease, in which the peripheral joints, where the tendon attached to the bone, and joints of the pelvis and spine, are affected. Spondyloarthropathies are frequently associated with chronic inflammatory bowel disease. Calprotectin, protein commonly found in neutrophils, is one of the major proteins of the inflammatory response and defense against pathogens. Studies have shown that faecal calprotectin is a promising marker for monitoring the activities of inflammatory bowel disease and the success of therapy. The aim of this study was to determine if calprotectin could serve to predict and monitor the course of disease in patients with juvenile spondyloarthropathies. For this purpose, two methods were used for the detection of calprotectin in faeces, polyclonal ELISA Calprest kit and monoclonal EliA Calprotectin automated method. The results show a higher level of correlation between calprotectin, obtained by the first method, with other parameters of inflammation (erythrocyte sedimentation rate and C-reactive protein). However, based only on our results, faecal calprotectin can not yet be used as adequate biomarker in predicting the course of juvenile spondyloarthropathies.

• Keywords: artritis; spondiloartropatije; upala; kalprotektin
• Keywords (english): arthritis; spondyloarthropathies; inflammation; calprotectin
• Language: croatian
• URN:NBN: urn:nbn:hr:217:249388
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 11:52:03