Združeno djelovanje cisplatine i resveratrola na metaloproteinaznu aktivnost u Ehrlichovom ascitesnom solidnom tumoru miša

master's thesis

• Title (english): Combined effect of cisplatin and resveratrol on metalloproteinase activity in mouse Ehrlic ascites solid tumor
• Author: Dominik Pinjuh
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-02-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovog istraživanja bio je istražiti združeno djelovanje citostatika cisplatine i resveratrola na inhibiciju angiogeneze i razvoja tumora, povećanje životnog vijeka domaćina, te istražiti aktivnost makrofaga u tkivu tumora kroz analizu inducibilne dušik oksid sintaze. Uz to cilj je bio utvrditi kako višekratno djelovanje citostatika djeluje na razinu proteina toplinskog stresa i da li obrada resveratrolom modulira njihovu aktivnost vodeći dokidanju rezistencije tumorskih stanica na citostatik. Tumor je izazvan injiciranjem 1x106 stanica EAT subkutano (sc) u bedro miševa soja Swiss albino. Obrada životinja s EAT započeta je unosom resveratrola per os u dozi od 50 mg/kg tijekom 5 dana u nizu s početkom 2. dana od injiciranja tumora dok je cisplatina injicirana u dozi od 2,5 mg/kg 10. i 12. dana te dozom od 5 mg/kg 15. dana. Dobiveni rezultati pokazuju da resveratrol združen s cisplatinom može inhibirati proces angiogeneze, usporiti rast tumora te povećati životni vijek nositelja tumora jačanjem imunomodulatorne aktivnosti domaćina kroz modulaciju proteina toplinskog stresa HSP-90 i HSP-70 i smanjuje štetne učinke cisplatine na normalne stanice i tkiva, što ukazuje na mogućnost kliničke uporabe ove kombinacije

Abstract (english)

The aim of this study was to investigate the combined effect of cytostatic cisplatin and resveratrol on inhibition of angiogenesis and tumor development, increase of host lifespan, and to investigate macrophage activity in tumor tissue through analysis of inducible nitric oxide synthase. In addition, the aim was to determine how the repeated action of cytostatics affects the level of heat shock proteins and whether resveratrol treatment modulates their activity leading to the abolition of tumor cell resistance to cytostatics. The tumor was induced by injecting 1x106 EAT cells subcutaneously (sc) into the thighs of Swiss albino mice. Treatment of animals with EAT was initiated by administration of resveratrol per os at a dose of 50 mg/kg for 5 consecutive days beginning on day 2 after tumor injection while cisplatin was injected at a dose of 2.5 mg/kg on days 10 and 12 and at a dose of 5 mg/kg on day 15. The results show that cisplatin combined with resveratrol can inhibit angiogenesis, slow tumor growth, and increase host lifespan by enhancing host immunomodulatory activity by modulating heat shock proteins HSP-90 and HSP70 and reducing the adverse effects of cisplatin on normal cells and tissues, which suggest the possibility of clinical use of this combination.

• Keywords: solidni tumor; resveratrol; cisplatina; angiogeneza; kemorezistencija
• Keywords (english): solid tumor; resveratrol; cisplatin; angiogenesis; chemoresistance
• Language: croatian
• URN:NBN: urn:nbn:hr:217:588142
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-02-26
• Created on: 2021-03-09 07:59:05