Polimorfizam mikrosatelita HLA u parovima primatelj - davatelj pri transplantaciji krvotvornih matičnih stanica

master's thesis

• Title (english): HLA MICROSATELLITES POLYMORPHISM IN RECIPIENT-DONOR PAIRS IN HEMATOPOIETIC STEM CELL TRANSPLANTATION
• Author: Marina Rimac
• Mentor: Zorana Grubić (mentor)
• Committee member: Zorana Grubić (predsjednik povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-06-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ovom radu praćen je utjecaj šest mikrosatelita HLA (D6S1014, D6S273, TNFa, D6S2927, D6S2793, D6S510) na ishod transplantacije krvotvornih matičnih stanica (TKMS) odnosno preživljavanje bolesnika. U istraživanje je uključeno 50 parova primatelj-nesrodni davatelj kojima je izolirana DNA iz periferne krvi te određeni aleli mikrosatelitskih lokusa uz pomoć lančane reakcije polimerazom i elektroforeze na poliakrilamidnom gelu u automatskom sekvenceru. Mikrosatelit D6S2927 je pokazao podudarnost za oba alela kod najvećeg broja parova (91,5%). Jedna podudarnost, odnosno nepodudarnost za oba alela je u najvećem broju ispitivanih parova uočena za lokus TNFa (44,7% i 10,6%). Analiza preživljavanja i podudarnosti za svaki pojedini mikrosatelit pokazala je statističku značajnost samo za mikrosatelit D6S2793 (P<0,05). Bolesnici s dvije podudarnosti na mikrosatelitu D6S2793 statistički su značajno duže preživljavali u usporedbi s bolesnicima s jednom, odnosno niti jednom podudarnošću. Na temelju toga možemo pretpostaviti da je regija u neposrednoj blizini mikrosatelita D6S2793 važna za preživljavanje bolesnika nakon nesrodne TKMS.

Abstract (english)

In this thesis we analyzed the influence of six HLA microsatellites (D6S1014, D6S273, TNFa, D6S2927, D6S2793, D6S510) on the patients' survival after hematopoietic stem cells transplantation (HSCT). DNA of 50 recipient-unrelated donor pairs, included in the study, was isolated from peripheral blood. Microsatellites analysis was performed using polymerase chain reaction and electrophoresis on a polyacrylamide gel in an automated sequencer. D6S2927 microsatellite demonstrated a two-allele match for the majority of tested pairs (91.5%). Conversely, TNFa locus showed one-allele match and no match in most cases (44.7% and 10.6%, respectively). Analysis of allele matching association with patient's survival showed a statistical significance only for D6S2793 (P<0.05). Patients with two D6S2793 allele matches showed a better survival compared to patients with one or no allele matched. Based on this we can suggest that the region in the proximity of D6S2793 microsatellite is of importance to the patient's survival after unrelated HSCT.

• Keywords: transplantacija krvotvornih matičnih stanica; nesrodni davatelj; HLA mikrosatelitski lokusi; preživljavanje bolesnika
• Keywords (english): hematopoietic stem cell transplantation; unrelated donor; HLA microsatellites; patients' survival
• Language: croatian
• URN:NBN: urn:nbn:hr:217:732001
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 07:52:02