Razina izraženosti transkripcijskogfaktora LEF-1 u glioblastomima

master's thesis

• Title (english): ANALYSIS OF THE LEVEL OF EXPRESSION OF TRANSCRIPTION FACTOR LEF-1 IN GLIOBLASTOMAS
• Author: Viktorija Sukser
• Mentor: Nives Pećina-Šlaus (mentor)
• Mentor: Dubravka Hranilović (sumentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Nives Pećina-Šlaus (član povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-09-04, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Glioblastomi su najčešći i najagresivniji tumori mozga odraslih osoba, te se prema kriterijima Svjetske zdravstvene organizacije klasificiraju kao astrocitomi gradusa IV. Mogu nastati de novo (primarni) ili iz astrocitoma nižeg gradusa (sekundarni). Svaki posjeduje karakteristične genetičke promjene. Među signalnim putevima koje pogađaju ovi poremećaji nalazi se put Wnt, važan tijekom normalnog embrionalnog razvoja i u matičnim stanicama. U njemu je ključna molekula β-katenin: kada nema signala on se razgrađuje, a kada je put Wnt aktivan, tada β-katenin ulazi u jezgru i veže se za transkripcijske faktore iz obitelji TCF/LEF. Jedan od njenih članova je LEF-1 (lymphoid enhancer factor-1) – zajedno s β-kateninom pokreće transkripciju mnogih gena koji, ukoliko je signalizacija nepravilna, doprinose napretku i invazivnosti tumora. U ovom radu analizirana je razina izraženosti LEF-1 u uzorcima tkiva glioblastoma. Imunohistokemijskim metodama protein LEF-1 lokaliziran je u jezgrama stanica te je utvrđeno da je njegova ekspresija pojačana u 80,6% analiziranih uzoraka.

Abstract (english)

Glioblastomas are the most common and most aggressive brain tumours in adults, and are classified as grade IV astrocytoma by the criteria of the World Health Organization. They can develop either de novo (primary) or from a lower-grade astrocytoma (secondary). Each type has its own characteristic genetic changes. Among the signalling pathways affected by these disorders is the Wnt pathway, which is important during normal embryonic development, as well as in stem cells. The key molecule in this pathway is β-catenin: when signals are absent, it is degraded, and when the Wnt pathway is activated, then β-catenin enters the nucleus and binds to transcription factors of the TCF/LEF family. One of its members is LEF-1 (lymphoid enhancer factor-1) which, together with β-catenin, drives the transcription of many genes that, if the signalling is aberrant, contribute to progression and invasiveness of the tumour. This study analyses the level of expression of LEF-1 in glioblastoma tissue samples. With the use of immunohistochemical methods, protein LEF-1 was localized in the cells' nuclei and its expression was found to be elevated in 80,6% of analyzed samples.

• Keywords: transkripcijski faktor LEF-1; glioblastom; signalni put Wnt
• Keywords (english): transcription factor LEF-1; glioblastoma; Wnt pathway
• Language: croatian
• URN:NBN: urn:nbn:hr:217:334366
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 08:25:42