Ekotoksikološka procjena utjecaja kardiovaskularnih farmaceutika prisutnih u vodenim ekosustavima na embrije ribe zebrice Danio rerio (Hamilton, 1822)

master's thesis

• Title (english): Ecotoxicological assessment of the impact of cardiovascular pharmaceuticals present in aquatic ecosystems on zebrafish embryos Danio rerio (Hamilton, 1822)
• Author: Gabrijela Prgić
• Mentor: Goran Klobučar (mentor)
• Mentor: Sanja Babić (mentor)
• Committee member: Goran Klobučar (predsjednik povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Petar Kružić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-06-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Proces onečišćenja okoliša doživljava eksponencijalan i nezaustavljiv rast te je postao jedan od najvećih problema s kojima se danas čovječanstvo susreće. Napredak znanosti doveo je i novu vrstu onečišćenja, lijekovima, odnosno farmaceutski aktivnim spojevima koji su jedna od glavnih komponenti onečišćenja kopnenih voda i koji su utvrđeni u sedimentu i plazmi riba iz rijeke Save. Na temelju dosadašnjih istraživanja izdvojeni su kardiovaskularni lijekovi: amiodaron, ramipril, simvastatin i verapamil čiji je utjecaj istražen, pojedinačno i u smjesi, na razvoj embrija ribe zebrice Danio rerio (Hamilton, 1822) u prvih 96 hpf. Tijekom izlaganja okolišno relevantnim koncentracijama (0.1, 1.0 i 10.0 μg/L) praćene su izazvane promjene kao što su mortalitet i stopa abnormalnosti, dužina ličinki, stopa izvaljivanja, brzina otkucaja srca, spontani pokreti prsnim perajama i oksidativni stres. Povećanjem koncentracije i vremenske izloženosti jedinki testiranim spojevima uočeni su učinci pri izlaganju svim spojevima, no najveća toksičnost zabilježena je na uzorku simvastatina. Integracijom krajnjih bioloških učinaka ispitivanih unutar ovog istraživanja, potvrđena je izvrsna uzročno-posljedična veza između izlaganja farmaceutskim spojevima i zabilježenog učinka na akvatičkom organizmu, čime je pružena podloga za biomonitoring onečišćenja okoliša, posebice slatkovodnih ekosustava.

Abstract (english)

The process of environmental pollution is experiencing exponential and unstoppable growth and has become one of the biggest problems facing humanity today. Progress in science has also brought a new type of pollution, therapeutic drugs, or pharmaceutically active compounds that are one of the main components of inland water pollution and that have been found in the sediment and plasma of fish from the Sava River. Based on previous research, cardiovascular drugs have been chosen: amiodarone, ramipril, simvastatin and verapamil, the effect of which has been investigated, individually and in a mixture, on the development of the zebrafish embryo Danio rerio (Hamilton, 1822) in the first 96 hpf. During exposure to environmentally relevant concentrations (0.1, 1.0 and 10.0 μg/L), caused changes such as mortality and abnormality rate, larval length, hatching rate, heart rate, spontaneous movements of the pectoral fins and oxidative stress were monitored. With increasing concentration and time exposure of individuals to the tested compounds, effects on exposure to all compounds were observed, but the highest toxicity was observed on the simvastatin sample. The integration of the ultimate biological effects examined within this study confirmed the excellent cause-and-effect relationship between exposure to pharmaceutical compounds and the observed effect on the aquatic organism, thus providing a basis for biomonitoring of environmental pollution, especially freshwater ecosystems.

• Keywords: organska onečišćenja; kardiovaskularni lijekovi; vodeni ekosustavi; morfološke i fiziološke promjene; kardiotoksičnost; neurotoksičnost; Danio rerio
• Keywords (english): organic pollution; cardiovascular drugs; aquatic ecosystems; morphological and physiological changes; cardiotoxicity; neurotoxicity; Danio rerio
• Language: croatian
• URN:NBN: urn:nbn:hr:217:948364
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-06-17
• Created on: 2021-06-23 14:09:45