Kvantitativno određivanje glifosata i njegovih metabolita vezanim sustavom tekućinska kromatografija-spektrometrija masa u hrani životinjskog podrijetla

doctoral thesis

• Title (english): Quantitative determination of glyphosate and its metabolites by liquid chromatography-mass spectrometry in food of animal origin
• Author: Marija Denžić Lugomer
• Mentor: Nina Bilandžić (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Nina Bilandžić (član povjerenstva)
• Committee member: Ivana Kmetič (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2021-06-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Razvijena je metoda za kvantitativno određivanje ostataka glifosata i njegovih metabolita AMPA, N-acetil-AMPA i N-acetil-glifosat vezanim sustavom tekućinska kromatografija spektrometrija masa u masnom tkivu, jetri, jajima, mlijeku i medu. Analiti su ekstrahirani iz uzoraka sa 1 % mravljom kiselinom u vodi (v/v). Predloženi analitički postupci validirani su u skladu sa smjernicama SANTE/12682/2019 pri čemu su provedene validacije na svim matricama kroz određivanje granica kvantifikacije, preciznosti i točnosti. Preciznost, izražena kao relativna standardna devijacija, bila je <20 %, dok je točnost, izražena kao analitički povrat, bila u rasponu od 70 % do 120 %. U okviru validacije metoda procijenjena je mjerna nesigurnost koja je bila <50 % za sve analite. Predloženim analitičkim postupcima analizirano je 60 uzoraka hrane životinjskog podrijetla.

Abstract (english)

A method for the quantitative determination of glyphosate residues and its metabolites AMPA, N-acetyl-AMPA and N-acetyl-glyphosate by liquid chromatography - mass spectrometry in fat tissue, liver, eggs, milk and honey was developed. Analytes were extracted from samples with 1% formic acid in water (v/v). The proposed analytical procedures were validated in accordance with the guidelines SANTE / 12682/2019, where validations were performed on all matrices by determining the limits of quantification, precision and accuracy. Precision, expressed as relative standard deviation, was <20%, while accuracy, expressed as analytical recovery, ranged from 70% to 120%. As part of the method validation, the measurement uncertainty was estimated to be <50% for all analytes. The proposed analytical procedures were used to analyze 60 samples of food of animal origin.

• Keywords: AMPA; glifosat; hrana životinjskog podrijetla; LC-MS/MS; N-acetil-AMPA; N-acetil-glifosat
• Keywords (english): AMPA; glyphosate; food of animal origin; LC-MS/MS; N-acetyl-AMPA; N-acetyl-glyphosate
• Language: croatian
• URN:NBN: urn:nbn:hr:217:786969
• Promotion: 2021
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: 106 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-06-16
• Created on: 2021-06-24 11:28:10