Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica na području Skadra i Boke Kotorske

master's thesis

• Title (english): Diversity of Cryphonectria parasitica vegetative compatibility types in Skadar and Boka Kotorska region
• Author: Tena Šibenik
• Mentor: Mirna Ćurković Perica (mentor)
• Committee member: Mirna Ćurković Perica (predsjednik povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cryphonectria parasitica je uzročnik raka kore kestena u šumama Europe i Sjeverne Amerike. Mikovirus Cryphonectria hypovirus 1 (CHV1) uzrokuje hipovirulenciju gljive C. parasitica i djeluje kao uspješno sredstvo biološke kontrole raka kore kestena. Prijenos virusa je ograničen sustavom vegetativne (ne)kompatibilnosti (vc) gljive, koji u Europi uključuje najmanje šest poznatih dialelnih lokusa (vic lokusi). Učinkovitost prijenosa virusa ovisi o raznolikosti tipova vegetativne kompatibilnosti gljive na određenom području. Cilj ovog istraživanja bio je odrediti raznolikost vc tipova populacije C. parasitica na području Boke kotorske i jezera Skadar u Crnoj Gori. U dvije regije u Crnoj Gori utvrđeno je 9 vc tipova unutar 79 izolata gljive C. parasitica klasičnom i molekularnom metodom. U Boki kotorskoj dominatan vc tip bio je EU-12 što predstavlja 49% ukupnog broj izolata, zatim slijedi EU-2 što čini 14% , EU-8 (8%) dok se EU-17 i EU-22 pojavljuju manje učestalo, svaki sa zastupljenošću od 2%. Za 28% izolata gljive C. parasitica s područja Boke kotorske nije određen vc tip. Najzastupljeniji vc tip na području jezera Skadar je EU-12 (58%), zatim EU-11 (8%) i EU-10 (6%), dok se EU-1 i EU-6 pojavljuju manje učestalo, svaki sa zastupljenošću od 3%. U populaciji gljive s područja jezera Skadar nije bilo moguće odrediti vc tip za 22% izolata. Najrasprostranjeniji vc tip na području Crne Gore je tip EU-12 kao i u drugim državama južne i jugoistočne Europe.

Abstract (english)

Cryphonectria parasitica is the causal agent of chestnut blight in European forests. Mycovirus Cryphonectria hypovirus 1 (CHV1) causes hypovirulence of C. parasitica and acts as a succesful biocontrol agent of the chestnut blight. Virus transmission is limited by a vegetative (in)compatibility (vc) system of the fungus, which in Europe involves at least six diallelic vic loci. The effectiveness of virus transmission depends on the diversity of vegetative compatibility types of the fungus in a particular area. The aim of this research was to determine the diversity of vc types in C. parasitica populations from Bay of Kotor and lake Skadar in Montenegro. In these two regions in Montenegro the total of 9 vc types were identified among 79 isolates of C.parasitica by classical and molecular method. The dominant vc type in Bay of Kotor was EU-12 (49 %), followed by EU-2 ( 14%) and EU-8 (8%), while EU-17 and EU-22 appeared less frequently (each with 2%). The vc type of 28% of isolates from Bay of Kotor could not have been determined. The most common vc type at lake Skadar was EU-12 (58%), followed by EU-11 (8%) and EU-10 (6%). EU-1 and EU-6 appeared less frequently (each with 3%). The vc type of 22% of isolates from lake Skadar could not have been determined. The most widespread vc type in Montenegro is EU-12, the same as in other southern and southeastern European countries.

• Keywords: Cryphonectria parasitica; rak kore kestena; vegetativna (ne)kompatibilnost (vc)
• Keywords (english): Cryphonectria parasitica; chestnut blight; vegetative (in)compatibility (vc)
• Language: croatian
• URN:NBN: urn:nbn:hr:217:416716
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-14 13:04:37