Potencijal funkcionalnih skupina praživotinja u aktivnom mulju kao indikatora učinkovitosti pročišćavanja otpadnih voda

doctoral thesis

• Title (english): Potential of protozoan functional groups in activated sludge as an indicator of wastewater treatment efficiency
• Author: Mirjana Kepec
• Mentor: Renata Matoničkin Kepčija (mentor)
• Committee member: Tomislav Ivanković (predsjednik povjerenstva)
• Committee member: Tibela Landeka Dragičević (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; Zagreb
• Defense date and country: 2021-11-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Sastav zajednica protozoa i mikro-metazoa motren je u aktivnom mulju uređaja za pročišćavanje otpadnih voda u korelaciji s učinkom pročišćavanja. Istražena su dva režima rada uređaja kroz više mjeseci: pročišćavanje komunalne otpadne vode (K) i miješane komunalne i industrijske otpadne vode (K+I). Određen je biotički indeks aktivnog mulja (SBI) od 6 do 10, koji je indicirao dobro stanje aktivnog mulja i dobru učinkovitost pročišćavanja. Fizikalno-kemijski (električna vodljivost, suspendirane tvari, KPK, BPK5) i mikrobiološki parametri (ukupan broj bakterija, koliformne bakterije, Escherichia coli, sulfit-reducirajuće bakterije, Pseudomonas aeruginosa i kvasci) ukazali su na visoku učinkovitost pročišćavanja, uglavnom ˃95 %, uz iznimku pojedinih bakterija (enterokoki) i hranjivih soli. Sastav mikrobnih zajednica razlikovao se između dva režima rada, a stanje aktivnog mulja bilo je povoljnije tijekom K+I režima. U aktivnom mulju određeno je 38 svojti praživotinja i sitnih beskralježnjaka, uz dominaciju trepetljikaša (27 svojti), posebice svojti Peritrichia. Potvrđena je korelacija ključnih indikatorskih skupina s parametrima influenta i efluenta te s učinkovitosti pročišćavanja. Pristup baziran na pojedinim svojtama ukazao je na visoki indikatorski potencijal vrsta Vorticella convalaria, Vorticella microstoma, Opercularia coarctata i Aspidisca cicada.

Abstract (english)

Protozoan and micro-metazoan assemblages were investigated in activated sludge of wastewater treatment facility in correlation with wastewater purification efficiency. Two different campaigns were analysed through several months: purification of municipal wastewater (K) and combined municipal and industrial wastewater (K+I). Sludge biotic index (SBI) was between 6 and 10, indicating good status of activated sludge and good purification efficiency. Physico-chemical (elektrical conductivity, suspended solids, COD, BOD5) and microbiological parameters (total bacteria, coliforms, Escherichia coli, sulfit-reducing bacteria, Pseudomonas aeruginosa and yeasts) also indicated high purification efficiency, mostly > 95 %, with the exception of certain bacteria (enterococci) and nutrients. Microbial assemblage composition differed between the two campaigns, and the activated sludge status was better during K+I campaign. There were 38 taxa of protozoa and micro-metazoa in activated sludge, with the domination of ciliates (27 taxa), especially Peritrichia. Key indicator groups correlated well with purification efficiency and with influent and effluent parameters. Species-specific approach indicated high indicator potential of several taxa: Vorticella convalaria, Vorticella microstoma, Opercularia coarctata and Aspidisca cicada.

• Keywords: protozoa; trepetljikaši; biotički indeks aktivnog mulja; indikatorske vrste
• Keywords (english): protozoa; ciliates; Sludge Biotic Index; indicator species
• Language: croatian
• URN:NBN: urn:nbn:hr:217:228441
• Promotion: 2021
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-12-01 10:21:02