Učinak proteina, ugljikohidrata i ulja na bioraspoloživost polifenola i biološke učinke ekstrakta lista vrste Ginkgo biloba L.

master's thesis

• Title (english): The effect of proteins, carbohydrates and oils on bioavailability of polyphenols and bioactivities of Ginkgo biloba L. leaf extract
• Author: Ema Nikša
• Mentor: Gordana Rusak (mentor)
• Committee member: Gordana Rusak (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Tajana Begović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ginkgo biloba L. biljna je vrsta iz porodice Ginkgoaceae i poznata je po imenu „Ginko“ ili prema lokalnim imenima „Yin-xing“ u Kini i „Icho“ u Japanu. Ekstrakt lišća ginka koristi se u fitoterapiji i kao dodatak prehrani. Ima utjecaj na poboljšanje cerebralne cirkulacije, na mršavljenje, antidiabetički, antihipertenzitivni i hipolipemični učinak zbog čega se koriste za liječenje kardiovaskularnih bolesti. Cilj ovog rada je ispitati učinak proteina kazeina, glukoze i ulja na biodostupnost bioaktivnih tvari (fenola, flavonoida, fenolnih i hidroksicimetnih kiselina, flavanola, flavonola, proantocijanidina, terpenskih trilaktona) te na antioksidacijsku i antidijabetičku aktivnost i pomoć pri mršavljenu ekstrakta ginka u uvjetima probave in vitro. Koristeći spektrofotometrijske metode za određivanje koncentracije fitokemikalija, aktivnosti lipaze, α-amilaze, α-glukozidaze, glikacije albumina goveda i antioksidacijske aktivnosti, te analizu uzoraka ginka kromatografijom visoke moći razlučivanja i statističke metode (jednosmjerna ANOVA, Duncan test i analizu glavnih komponenti) utvrdila sam da primjena tableta ginka na prazan želudac ili uz hranu te sastav te hrane ovise o tome koji biološki učinak prvenstveno želimo postići. Što znači da treba imati drugačiji pristup u kombinaciji tableta ginka i hrane ako se radi o poboljšanju cerebralne cirkulacije i pamćenja, antioksidativnom ili antidijabetičkom učinku ili pomoći pri mršavljenju.

Abstract (english)

Ginkgo biloba L. is a plant species from Ginkgoaceae family, and is famous by name „Ginko“ or by local names „Yin-xing“ in China and „Icho“ in Japan. Extract of ginko leves is used in phitotheraphy and as a dietary supplemnet. It has effect on cerebral circulation, weight loss and antidiabetic, antihypertensive and hipolipemic effect wherefore is used for cardiovascular disease treatment. The main purpose of this thesis is to evaluate the effect of proteins, carbohydrates and oils on bioavailability of bioactive compounds (phenols, flavonoids, phenolic and hidroksi cinnamic acids, flavanoids, flavonols, proanthocyanidins, triterpene lactones), antioxidant and antidiabetic effects, and weight loss effect of ginko leaves extracts in conditions of digestion in vitro. Using spectrophotometric methods to determine the concentration of phytochemicals, lipase, α-amylase and α-glucosidase activity, BSA glycation and antioxidant activity, HPLC analysis as well as statistical methods (one-way ANOVA, Duncan test and principal component analysis), I have confirmed that the use of ginkgo tablets on an empty stomach or with food, as well as the composition of these foods, depend on which biological effect we primarily want to achieve. Therefore, a different approach should be taken when combining ginkgo tablets with food.

• Keywords: ginko; biodostupnost; antioksidativna aktivnost; antidijabetička aktivnost; mršavljenje
• Keywords (english): ginkgo; bioavailability; antioksidant; antidijabetic activity; weight loss
• Language: croatian
• URN:NBN: urn:nbn:hr:217:012426
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-02-28
• Created on: 2022-03-17 18:08:54