Metabolomski pristupi u otkrivanju novih biomarkera i dubinska fenotipizacija u bubrežnim i srčanim bolestima u pasa

doctoral thesis

• Title (english): Metabolomic approaches to novel biomarker discovery and deep phenotyping in renal and cardiac diseases in dogs
• Author: Ivana Rubić
• Mentor: Vladimir Mrljak (mentor)
• Committee member: Marko Močibob (predsjednik povjerenstva)
• Committee member: Vlasta Drevenkar (član povjerenstva)
• Committee member: Renata Barić-Rafaj (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2022-06-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Babezioza i dilatacijska kardiomiopatija važne su bolesti koje se nalaze u pasa, ali i ljudi. Jedna je od najčešćih komplikacija kod babezioze pasa uzrokovane protozoonom Babesia canis akutna ozljeda bubrega. Drugi je po učestalosti uzrok zatajenja srca u pasa idiopatska dilatacijska kardiomiopatija (iDCM) koja rezultira zatajenjem srca ili iznenadnom srčanom smrću zbog nastanaka aritmije. Svrha je istraživanja bila otkriti promjene u metabolomu seruma i urina pasa s promijenjenom funkcijom bubrega kod babezioze, te u metabolomu plazme pasa s iDCM-om u odnosu na zdrave pse. U istraživanju su primijenjeni neciljani i ciljani metabolomski pristup upotrebom tekućinske kromatografije spregnute sa spektrometrijom masa te metabolomska analiza temeljena na plinskoj kromatografiji spregnutoj sa spektrometrijom masa. U metabolomu seruma i urina pasa s babeziozom otkrivene su promjene povezane s oksidacijskim stresom, upalnim odgovorom domaćina, hipoksijom, anemijom i energetskim metabolizmom. U istraživanju iDCM-a identificirane su promjene u metabolomu plazme pasa i poremećaji u metaboličkim putevima koji su uključeni u
razvoj iDCM-a.

Abstract (english)

Babesiosis and dilated cardiomyopathy are important diseases in dogs and humans. Acute kidney injury is one of the most common complications in canine babesiosis caused with protozoa Babesia canis. The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. This study aimed to determine changes in the serum and urine metabolome of dogs with renal failure in babesiosis, and in the plasma metabolome of dogs with iDCM compared to healthy dogs. In this study, we applied an untargeted and targeted metabolomics approach using liquid chromatography coupled to mass spectrometry and gas chromatography coupled to mass spectrometry-based metabolomics. In the serum and urine samples of dogs with babesiosis were found differences associated with oxidative stress, inflammatory host response, hypoxia, anemia, and energy metabolism. We discovered changes in canine plasma metabolome and metabolic pathway disorders involved in the development of iDCM.

• Keywords: babezioza; idiopatska dilatacijska kardiomiopatija; metabolomika; plinska kromatografija; tekućinska kromatografija; spektrometrija masa
• Keywords (english): babesiosis; idiopathic dilated cardiomyopathy; metabolomics; gas chromatography; liquid chromatography; mass spectrometry
• Language: croatian
• URN:NBN: urn:nbn:hr:217:971002
• Promotion: 2022
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: LXXXVII, 149 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-06-30 14:36:34