Stabilnost i toksični učinci specija arsena u uzorcima voda

doctoral thesis

• Title (english): Stability and toxic effects of arsenic species in water samples
• Author: Amra Alispahić
• Mentor: Adela Krivohlavek (mentor)
• Mentor: Nives Galić (komentor)
• Committee member: Iva Juranović Cindrić (predsjednik povjerenstva)
• Committee member: Ivone Jakaša (član povjerenstva)
• Committee member: Gordana Pehnec (član povjerenstva)
• Committee member: Sanda Rončević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2022-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

U ovom radu ispitana je stabilnost omjera anorganskih specija arsena As(III)/As(V) u modelnim otopinama i uzorcima prirodnih podzemnih voda s četiri lokacije u Hrvatskoj uz dodatak sljedećih konzervansa: limunska kiselina, natrijev citrat, kalijev oksalat, kalijev–natrijev tartarat i octena kiselina. Specijacijska analiza provedena je metodom voltametrije anodnog otapanja i UPLC–ICP–MS. Utvrđeno je kako kombinacija ispitanih konzervansa s octenom kiselinom uspješno stabilizira omjer specija arsena te je on u modelnim otopinama stabilan do 8 dana, dok je u uzorcima prirodnih voda stabilan 6 do 7 dana. Akutnim testom ekotoksičnosti određena je vrijednost LC50 nakon 24 h na Daphnia magna. Dobivene vrijednosti su 5,07 ppm za As(III), 10,27 ppm za As(V) i 0,58 ppm za As(III) s nanočesticama željeza. MTT testom citotoksičnosti na HepG2 staničnoj liniji određena je vrijednosti LC50 nakon 24 h. Najveća citotoksičnost određena je za As(III) uz dodatak SPIO nanočestica, dok As(V) ne inducira staničnu smrt.

Abstract (english)

In this paper, the stability of inorganic arsenic species ratio As(III)/As(V) in model solutions and natural groundwater samples from four locations in Croatia was investigated. Selected preservatives were citric acid, sodium citrate, potassium oxalate, potassium sodium tartrate and acetic acid. Speciation analysis was performed by anodic stripping voltammetry method and
UPLC–ICP–MS. It was shown that the combination of the tested preservatives with acetic acid stabilize the arsenic species ratio for up to 8 days in model solutions and for 6 to 7 days in natural water samples. The LC50 values determined by acute ecotoxicity test on Daphnia magna after 24 h amounted to 5.07, 10.27 and 0.58 ppm for As(III), As(V), As(III)/iron nanoparticles, respectively. Cytotoxicity was determined by MTT test on human hepatocarcinoma cell line (HepG2) after 24 h and expressed as LC50 value. The most pronounced effect was observed for As(III)/Fe nanoparticles, while As(V) was inactive.

• Keywords: citotoksičnost; ekotoksičnost; specije arsena; stabilnost; voda; voltametrija; UHPLC–ICP–MS
• Keywords (english): arsenic species; cytotoxicity; ecotoxicity; stability investigation; UHPLC–ICPMS– voltammetry; water
• Language: croatian
• URN:NBN: urn:nbn:hr:217:885388
• Promotion: 2022
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: XLIX, 128 str. ; 30 cm
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-07-12
• Created on: 2022-07-12 16:33:16