Utjecaj razine kisika na razvoj humanih zametaka u kulturi in vitro

master's thesis

• Title (english): Effect of diverse oxygen levels on the human embryo development during embryo culture
• Author: Sonja Šogorić Ševo
• Mentor: Vesna Benković (mentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Inga Marijanović (član povjerenstva)
• Committee member: Anđelka Plenković-Moraj (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-10-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

S obzirom na broj djece začete medicinski potpomognutom oplodnjom (MPO), ova metoda je vrlo važan izbor liječenja i povećanja vjerojatnosti trudnoće bračnih parova s problemom neplodnosti. Pretpostavlja se da je kisik, osim sastava medija za kultivaciju, ključan čimbenik u razvoju zametaka in vitro, jer uzrokuje nastanak reaktivnih kisikovih radikala koji se smatraju glavnim čimbenicima odgovornima za nezadovoljavajuće rezultate MPO. Cilj istraživanja je bio usporediti građu ljudskih zametaka od oplodnje do prijenosa zametaka u maternicu tijekom in vitro kultivacije u uvjetima uzgoja od 21% i 5% kisika sa stupnjem kliničkih trudnoća. Zameci kultivirani u inkubatorima sa 5% kisika u većem se udjelu razvijaju do stadija blastociste i rezultiraju većim brojem trudnoća. Kultivacija zametaka u atmosferi s 5% kisika pridonosi njihovom sveukupnom boljem razvoju.

Abstract (english)

Considering the number of children conceived through in vitro fertilization (IVF), assisted reproduction is a very important choice of treatment because it increases the probability of pregnancy in couples with infertility problems. It is assumed that oxygen, along with the composition of the media culture, is a key factor in development of embryos in vitro, becouse it causes the formation of reactive oxygen species, which are considered the main factors responsible for the unsatisfactory results of IVF. The goal of the research was to compare the structure of human embryos from fertilization to embryo transfer into the uterus during in vitro cultivation in conditions with oxygen levels of 21% and 5% with the degree of clinical pregnancies. Embryos cultured in incubators with 5% level of oxygen are more likely to reach the blastocyst stage and resulted in a larger number of pregnancies. Cultivation of embryos in incubators with 5% oxygen contributes to their overall better development.

• Keywords: medicinski potpomognuta oplodnja; 5% kisika; razvoj zametaka; blastocista
• Keywords (english): in vitro fertilization; 5% oxygen; embryo development; blastocysts
• Language: croatian
• URN:NBN: urn:nbn:hr:217:079902
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 11:59:01