Okolišna DNA u istraživanju mora

undergraduate thesis

• Title (english): Environmental DNA in sea research
• Author: Tena Ćurko
• Mentor: Tatjana Bakran-Petricioli (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Okolišna DNA (eDNA) može biti definirana kao rezervoar DNA prisutan u okolišnom uzorku koji
uključuje izvanstaničnu i unutarstaničnu DNA. To je genetski materijal sakupljen iz okolišnog
uzorka koji ne sadrži razlikovne značajke izvornih makro-organizama. Potencijalni izvori eDNA
su svi biološki materijali organizama, a razgradnja eDNA ovisi o različitim abiotičkim uvjetima.
eDNA je od izrazite važnosti u istraživanjima bioraznolikosti okoliša, jer je analiza eDNA
jednostavna i jeftina te omogućuje kvantitativne procijene biomase i brojnosti jedinki i vrsta.
Promijene okolišnih uvjeta utječu na okolišnu DNA zbog čega se analiza eDNA često koristi u
ekologiji. Osim klimatskih promjena tu su i sezonske promjene abiotičkih čimbenika koje značajno
utječu na degradaciju eDNA. Mediteran je mjesto izrazite bioraznolikosti istraživane
tradicionalnim i modernim metodama. U moderno doba ta bioraznolikost ugrožena je
neautohtonim vrstama čije je praćenje olakšano analizom okolišne DNA.

Abstract (english)

Environmental DNA (eDNA) can be defined as the DNA pool recovered from an environmental
sample that includes both extracellular and intracellular DNA. Here, eDNA is defined as the genetic
material obtained from a water sample containing no distinguishing characters of original macroorganisms. Potential sources of eDNA are all the biomaterials of organisms. Degradation of eDNA
depends on different abiotic conditions. eDNA analysis is of great importance in research and
determination of environmental biodiversity because it is a simple and cost-effective method that
also enables quantitative assessment of biomass and abundance of individuals and species. Changes
in environment reflect in environmental DNA and therefore the eDNA analysis is often used in
ecology. Besides climate change there are also seasonal changes of abiotic factors that significantly
affect degradation of eDNA. The Mediterranean is a hotspot of biodiversity, researched using
traditional and modern methods. In modern times this biodiversity is threatened by non-indigenous
species whose monitoring is facilitated with environmental DNA analysis.

• Keywords: okolišna DNA; bioraznolikost; biomonitoring; Mediteran; sekvenciranje
• Keywords (english): environmental DNA; biodiversity; biomonitoring; Mediterranean; sequencing
• Language: croatian
• URN:NBN: urn:nbn:hr:217:546266
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-05 09:35:49